Skip to main content
SpringerLink
Log in

Liver regeneration following partial hepatectomy and stimulation by hepatic stimulatory substance in cirrhotic and non-cirrhotic rats

  • Original Papers
  • Published:
Research in Experimental Medicine

Abstract

The effects of hepatic stimulatory substance (HSS) on cirrhotic and noncirrhotic rats were studied after 70% partial hepatectomy. Liver cirrhosis was produced by weekly intragastric infusion of chloroform for 12–16 weeks. The HSS was prepared by extraction from the livers of weanling mice. Rats in the experimental group were injected with 5 ml HSS after 70% partial hepatectomy, and those in the control group received normal saline. The results showed that the3H-thymidine incorporation was higher in the HSS group 24 h after partial hepatectomy in both cirrhotic and non-cirrhotic rats, and persistently higher in the non-cirrhotic rats at 48h. Total DNA was significantly higher in the HSS group of non-cirrhotic rats 24 and 48 h after partial hepatectomy. The restituted liver volume and weight was significantly higher in non-cirrhotic rats 48 h after partial hepatectomy, while there was no significant difference between the HSS and the control groups in the cirrhotic rats. The HSS induced significant effects on3H-thymidine incorporation in the non-cirrhotic liver, resulting in increasing liver weight, volume and total DNA 48 h after partial hepatectomy. In cirrhotic rats, the3H-thymidine incorporation was higher in the HSS group at 24 h after partial hepatectomy, though not showing any increase at 48 h, but the regeneration of liver weight, volume and total DNA at 48 h showed no difference between the HSS group and the control group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bucher NLR, Malt RA (1971) Regeneration of the liver and kidney. Little, Brown, Boston

    Google Scholar 

  2. Bucher NLR (1982) Thirty years of liver regeneration: a distillate. In: Sato GH, Pardee AB, Sirbasku DA (eds) Cold Spring Harb Symp Quant Biol 9: 15–24

  3. Hoorn-Hickman RV, Kahn D, Green J, Macleod HA, Terblanche J (1981) Is there a regeneration stimulator substance in the effluent from perfused partially hepatectomized livers? Hepatology 1: 287–293

    Article  PubMed  Google Scholar 

  4. Fleig WE, Hoss G (1989) Partial purification of rat hepatic stimulator substance and characterization of its action on hepatoma cells and normal hepatocytes. Hepatology 9: 240–248

    Article  PubMed  CAS  Google Scholar 

  5. LaBrecque DR, Steele G, Fogerty S, Wilson M, Barton J (1987) Purification and physicalchemical characterization of hepatic stimulator substance. Hepatology 7: 100–106

    Article  PubMed  CAS  Google Scholar 

  6. Zarnegar R, Michalopoulos G (1989) Purification and biological characterization of human hepatopoietin A: a polypeptide growth factor for hepatocytes. Cancer Res 49: 3314–3320

    PubMed  CAS  Google Scholar 

  7. Morley CGD, Kingdon HS (1973) The regulation of cell growth. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats. Biochim Biophys Acta 308: 260–275

    PubMed  CAS  Google Scholar 

  8. LaBrecque DR, Wilson M, Fogerty S (1984) Stimulation of HTC hepatoma cell growth in vitro by hepatic stimulator substance (HSS): interaction with serum, glucagon, epidermal growth factor and platelet-derived growth factor. Exp Cell Res 150: 419–429

    Article  PubMed  CAS  Google Scholar 

  9. LaBrecque DR, Pesch LA (1975) Preparation and partial characterization of hepatic regenerative stimulator substance from rat liver. J Physiol 248: 272–284

    Google Scholar 

  10. Starzl TE, Terblanche J, Porter KA, Jones AF, Usui S, Mazzoni G (1979) Growth-stimulating factor in canine liver. Lancet 1: 127–130

    Article  PubMed  CAS  Google Scholar 

  11. Terblanche J, Porter KA, Starzl TE, Moor J, Patzelt L, Hayashida N (1980) Stimulation of hepatic regeneration after 44% partial hepatectomy by infusion of a cytosol extract from dog liver. Surg Gynecol Obstet 151: 538–544

    PubMed  CAS  Google Scholar 

  12. Fleig WE, Lehmann H, Wagner H, Hoss G, Ditschuneit H (1986) Hepatic regeneration stimulator substance in the rabbit: relation to liver regeneration after partial hepatectomy. J Hepatol 3: 19–26

    Article  PubMed  CAS  Google Scholar 

  13. Nagasue N, Yukaya H, Ogawa Y, Nakamura T (1987) Human liver regeneration after major hepatic resection: a study of normal liver and livers with chronic hepatitis and cirrhosis. Ann Surg 206: 30–39

    Article  PubMed  CAS  Google Scholar 

  14. Chen MF, Hwang TL, Hung CF (1991) Human liver regeneration after major hepatectomy—A study of liver volume by computerized tomography. Ann Surg 213: 227–229

    Article  PubMed  CAS  Google Scholar 

  15. Proctor E, Chatamra K (1982) High yield micronodular cirrhosis in the rat. Gastroenterology 83: 1183–1190

    PubMed  CAS  Google Scholar 

  16. Chen MF, Hwang TL (1994) The regeneration of cirrhotic liver after partial hepatectomy: A study using the rat chloroform induced cirrhotic model. J Biochem Sci 18: 71–75

    CAS  Google Scholar 

  17. LaBrecque DR, Steele G, Fogerty S, Wilson M, Barton J (1987) Purification and physicalchemical characterization of hepatic stimulator substance. Hepatology 7: 110–116

    Article  Google Scholar 

  18. Higgins M, Anderson RM (1931) Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12: 186–202

    Google Scholar 

  19. Hwang TL, Chen MF, Chen TJ (1993) Augmentation of liver regeneration with glucagon after partial hepatectomy in rats. J Formos Med Assoc 92: 725–728

    PubMed  CAS  Google Scholar 

  20. Macintosh E, Gauthier T, Pettigrew N, Minuk G (1992) Liver regeneration and the effect of exogenous putrescine on regenerative activity after partial hepatectomy in cirrhotic rats. Hepatology 16: 1428–1433

    Article  PubMed  CAS  Google Scholar 

  21. Baskin G, Henderson GI, Schenker S (1988) Ethanol and hepatic regeneration. Hepatology 8: 408–411

    Article  PubMed  CAS  Google Scholar 

  22. Digernse V, Bronstad G, Sand TE, Christofferson T (1982) The proliferative response of rat liver parenchymal cells after partial hepatectomy: a methodological study comparing flow cytometry of nuclear DNA content in vivo and in vitro uptake of thymidine., Cell Tissue Kinet 15: 521–528

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Chang Gung Memorial Hospital, Chang Gung College of Medicine and Technology, 199, Tung-Hwa N Road, Taipei, Taiwan, ROC

    Tsann-Long Hwang, Hon-Chung Yu, Pu-Cheng Chen & Miin-Fu Chen

Authors
  1. Tsann-Long Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hon-Chung Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pu-Cheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Miin-Fu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hwang, TL., Yu, HC., Chen, PC. et al. Liver regeneration following partial hepatectomy and stimulation by hepatic stimulatory substance in cirrhotic and non-cirrhotic rats. Res. Exp. Med. 195, 201–208 (1995). https://doi.org/10.1007/BF02576789

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02576789

Key words

Search

Navigation