Abstract
Short term effects of ovulen-50, a combination type oral contraceptive agent and phenobarbital—an established hepatic tumour promoter, were examined in the livers of diethylnitrosamine-initiated and uninitiated female rats. Liver mitotic activity as judged by liver weight, [3H] thymidine incorporation into DNA and levels of DNA, RNA and protein were measured in non-regenerating and regenerating liver. Hepatic γ-glutamyl transpeptidase activity and hepatocyte agglutination with concanavalin A were examined in diethylnitrosamine- and/or phenobarbital-treated rats.
The results indicate that diethylnitrosamine or ovulen-50 individually are mitoinhibitory in regenerating liver. Phenobarbital alone has a slight mitostimulatory effects in non-regenerating liver, but no effect on liver regeneration. Administration of ovulen-50 and phenobarbital to diethylnitrosamine initiated rats mitigated the mitoinhibition during regeneration. Contrary to the earlier observation with ovulen-50, neither phenobarbital nor diethylnitrosamine induced hepatocyte agglutination in the presence of concanavalin A. Like ovulen-50, diethylnitrosamine also increased the level of hepatic γ-glutamyl transpeptidase. Phenobarbital produced only insignificant rise and did not substantially exacerbate the effect diethylnitrosamine.
The data show that though some of the effects of ovulen-50 resemble those of diethylnitrosamine or phenobarbital, the changes observed may not be related to the neoplastic phenomenon since they were not seen in an initiator-promoter combination regimen.
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Abbreviations
- OCA:
-
Oral contraceptive agent
- pH:
-
partial hepatectomy
- GGT:
-
γ-glutamyl transpeptidase
- Con A:
-
concanavalin A
- DEN:
-
diethylnitrosamine
- PB:
-
phenobarbital
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Annapurna, V.V., Mukundan, M.A., Sesikeran, B. et al. Effects of ovulen-50, diethylnitrosamine and phenobarbital on liver regeneration in female rats. J Biosci 14, 1–7 (1989). https://doi.org/10.1007/BF02703516
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DOI: https://doi.org/10.1007/BF02703516