Abstract
A cloned gene,Xa21 was transferred into five widely-used Chinese rice varieties through anAgrobacterium- mediated system, and over 110 independent transgenic lines were obtained. PCR and Southern analysis of transgenic plants revealed the integration of the wholeXa21 gene into the host genomes. The integratedXa21 gene was stably inherited, and segregated in a 3: 1 ratio in the selfed T1 generation when one copy of the gene was integrated in the transformants. Inoculation tests displayed that transgenic T0 plants andXa21 PCR-positive T1 plants were highly resistant to bacterial blight disease. The selectedXa21 homozygous resistant transgenic lines with desirable qualities may be propagated as new varieties or utilized in hybrid rice breeding.
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Zhai, W., Li, X., Tian, W. et al. Introduction of a rice blight resistance gene,Xa21, into five Chinese rice varieties through anAgrobacterium-mediated system. Sci. China Ser. C.-Life Sci. 43, 361–368 (2000). https://doi.org/10.1007/BF02879300
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DOI: https://doi.org/10.1007/BF02879300