Abstract
We have identified DNA polymorphisms in the gene of insulin-like growth factor 2 by PCR-SSCP in a resource population, which was generated by Silky reciprocally crossing to Broilers. A C →G mutation was detected in the exon 2 (at position 71) by sequencing. This single nucleotide polymorphism (SNP) was found to be associated with production traits. Chicken with BB genotype showed more chest angle width but less 3 week body weight and glandular stomach weight than chicken with AA genotype (P<0.05); while the heterozygote (AB genotype) chicken had more abdominal fat weight, eviscerated yield with giblet than AA homozygote chicken. Further analysis showed that there were different genetic effects on some traits between heterozygote AB (paternal allele given first) and heterozygote BA: chickens with genotype BA had more birth weight and breast weight but less abdominal fat weight than chickens with genotype AB (P<0.05), which could be hypothetically contributed by genome imprinting. Therefore, Silky chickens were selected for production of heterozygotes to confirm whether IGF2 locus was imprinting. Progeny from heterozygote × homozygote reciprocal cross was assayed for expression after the genotype was determined. The transcription of IGF2 was detected by RT-PCR-SSCP. IGF2 gene was expressed bialleleically in 1-day-old neonatal liver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens. Therefore, IGF2 was not an imprinting gene in chicken. The different genetic effects between the heterozygote AB and BA remain to be elucidated.
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Abbreviations
- W0:
-
weight of birth
- AF:
-
the abdominal fat weight
- AFR:
-
abdominal fat rate
- BW:
-
breast weight
- BWR:
-
breast weight ration
- SNP:
-
single nucleotide polymorphism
- IGF2:
-
insulin-like growth factor 2
- SSCP:
-
single strand conformation polymorphism
- RT:
-
reverse transcription
References
Rinderknecht, E., Humbel, R. E., The amino acid sequence of human insulin-like growth factor I and its structural homology withproinsulin, J. Biol. Chem., 1978, 253(8): 2769–2776.
Rinderknecht, E., Humbel, R. E., Primary structure of human insulin-like growth factor II, FEBS Lett., 1978, 89(2): 283–286.
Blundell, T. L., Bedarkar, S. Insulin-like growth factor: A model for tertiary structure accounting for immunoreactivity and receptor binding, Proc. Natl. Acad. Sci. USA, 1978, 75(1): 180–184.
Vasilatos, Y. R., Scanes, C. G., Growth hormone and insulin-like growth factor in poultry growth: Required, optimal or ineffective, Poultry Sci., 1991, 70: 1764–1780.
Lee, J. E., Pintar, J., Efstratiadis, A., Pattern of insulin-like growth factor II gene expression during early mouse embryogenesis, Development, 1990, 110: 151–159.
Stylianopoulou, F., Efstratiadis, A., Herbert, J., Pintar, J., Pattern of insulin-like growth factor II gene expression during rat embryogenesis, Development, 1988, 103: 497–506.
Dechiara, T. M., Robertson, E. J., Efstratiadis, A., Parental imprinting of the mouse insulin-like growth factor II gene, Cell, 1991, 64: 849–859.
McLaren, R. J., Montgomery, G. W., Genomic imprinting of the insulin-like growth factor 2 gene in sheep, Mammalian Genome, 1999, 10: 588–591.
Cockett, N. E., Jackson, S. P., Shay, T. L., Farnir, F., Berghmans, S., Snowder, G. D., Nielsen, D. M., Georges, M., Polaro overdominance at the ovinecallipyge locus, Science, 1996, 273: 236–238.
Schmidt, J. V., Matteson, P. G., Jones, B. K., Guan, X. J., Tilghman, S. M., The Dlk1 and Gtl2 genes are linked and reciprocally imprinted, Genes Cells, 2000, 14(16): 1997–2002.
Miyoshi, N., Wagatsuma, H., Wakana, S., Shiroishi, T., Nomura, M., Aisaka, K., Kohda, T., Surani, M. A., Kaneko-Ishino, T., Ishino, F., Identification of an imprinted gene, Meg3/Gtl2 and its human homologues MEG3, first mapped on mouse distsl chromosome 12 and human chromosome 14q, Genes Cells, 2000, 5(3): 211–220.
Charlier, C., Segers, K., Wagenaar, D., Karim, L., Berghmans, S., Jaillon, O., Shay, T., Weissenbach, J., Cockett, N., Gyapay, G., Georges, M., Human-ovine comparative sequencing of a 250-kb imprinted domain encompassing the callipyge (clpg) locus and identification of six imprinted transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and Meg8, Genome Res., 2001, 11: 850–862.
http://www.mgc.har.mrc.ac.uk/imprinting/implink.html
Moore, T., Haig, D., Genomic imprinting in mammalian development: A parental tug-of-war, Trends in Genetics, 1991, 7: 45–49.
O’Neill, M. J., Ingram, R. S., Vrana, P.B., Tilghman, S. M., Allelic expression of IGF2 in marsupials and birds, Dev. Genes Evol., 2000, 210: 18–20.
Nolan, C. M., Killian, J. K., Petitte, J. N., Jirtle, R. L., Imprint status of M6P/IGF2R and IGF2 in chickens, Dev. Genes Evol., 2001, 211: 179–181.
Yokomine, T., Kuroiwa, A., Tanaka, K., Tsudzuki, M., Matsuda, Y., Sasaki, H., Sequence polymorphism, allelic expression status and chromosome locations of the chicken IGF2 and MPR1 genes, Cytogenet. Cell Genet., 2001, 93: 109–113.
Koski, L. B., Sasaki, E., Roberts, R. D., Gibson, J., Etches, R. J., Monoalleleic transcription of the insulin-like growth factor2 gene (IGF2) in chicken embryos, Mol. Repro. Dev., 2000, 56: 345–352.
Fairfull, R. W., Gowe, R. S., Emsley, J. A., Diallel cross of six long-term selected leghorn strains with emphasis on heterosis and reciprocal effects, Br. Poult. Sci., 1983, 24: 133–158.
Darling, D. C., Brickell, P.M., Nucleotide sequence and genomic structure of the chicken insulin-like growth factor-II (IGF-II) coding region, General and Comparative Endocrinology, 1996, 102: 283–287.
Joen, J. T., Carlborg, Ö., Tornsten, A., Giuffra, E., Amarger, V., chardon, P., Lena, A. E., Andersson, K., Hansson, I., Lundstrom, K., Andersson, L., A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus, Nature Genetics, 1999, 21: 157–158.
DeChiara, T. M., Efstratiadis, A., Robertson, E. J., A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting, Nature, 1990, 345: 78–80.
Chess, A., Expansion of the Allelic Exclusion Principle? Science, 1998, 279: 2067–2068.
Blagitko, N., Mergenthaler, S., Schulz, U., Wollmann, H. A., Cralgen, W., Eggermannm, T., Ropers, H. H., Kalscheuer, V. M., Human GRB10 is imprinted and expressed from the paternal and maternal allele in a highly tissue- and isoform-specific fashion, Human Molecular Genetics, 2000, 9: 1587–1595.
Surani, M. A. H., Barton, S. C., Norris, M. L., Development of reconstituted mouse eggs suggests imprinting of the genome during gametogenesis, Nature, 1984, 308: 548–550.
McGrath, J., Sotter, D., Completion of mouse embryogenesis requires both the maternal and paternal genomes, Cell, 1984, 37: 176–183.
Ferguson-Smith, A. C., Suran, M. A., Imprintint and the epigenetic asymmetry between parental genomes, Science, 2001, 293: 1086–1089.
Olsen, M. W., Weights of some internal organs and glands of fully developed parthenogenetic and normal turkey embryos, Poultry Sci., 1970, 49: 1733–1735.
Olsen, M. W., Influence of turkey sires and dams on the level of parthenogenesis in eggs of their daughters, Poultry Sci., 1973, 52: 666–670.
Cassar, G., John, T. M., Etches, R. J., Observations on ploidy of cells and on reproductive performance in parthenogenetic turkeys, Poultry Sci., 1998, 77: 1457–1462.
Hurst, L. D., McVean, G. T., Growth effects of uniparental disomies and the conflict theory of genomic imprinting, Trends in Genetics, 1997, 13: 436–443.
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Wang, G., Yan, B., Deng, X. et al. Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken. Sci. China Ser. C.-Life Sci. 48, 187–194 (2005). https://doi.org/10.1007/BF02879672
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DOI: https://doi.org/10.1007/BF02879672