Abstract
Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same individuals were compared with virus carriage. In the periodontitis group, 60.5 %, and in the periodontosis group 61.5 % of patients carried viruses, while this was established only in 21.1 % of control cases. On the other hand, emigration and sulcular gathering of the less viable polymorphonuclear leukocytes was elevated but their phagocytotic activity was decreased among periodontitis patients. Bactericidal capacity was significantly lowered among those subjects who carried viruses in their cells, as compared with virusfree persons, especially in the periodontitis group. The functions of the polymorphonuclear leukocytes accumulated in the sulcus gingivalis may be modified by mediators released from the virus-carrying cells. These mediators could achieve a greater concentration locally, and the damaged leukocytes would not be able to eliminate the microbes continuously so that the accumulation of bacterial products, among them endotoxins, could lead to periodontal inflammation.
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Andiman W.A., Jacobson R.I., Tucker G.: Leukocyte-associated viraemia with adenovirus type 2 in an infant with lower-respiratory tract disease.New Engl.J.Med. 297, 100–101 (1977).
Baum S.G.: Persistent adenovirus infection of nonpermissive monkey cells.J.Virol. 23, 412–420 (1977).
Behling U.H., Sallay C., Sanavi F., Pham P.H., Nowotny A.: Humoral immunity and reduced periodontal bone loss inEikenella corrodens-monoassoeiated rats.Infect.Immun. 33, 801–805 (1981).
Chandler D.C., Silverman M.S., Lundblad R.L., McFall W.T.: Human parotid IgA and periodontal disease.Arch.Oral Biol. 19, 733–735 (1974).
Cianciola L.J., Genco R.J., Patters M.R., McKenna J., van Oss C.J.: Defective polymorphonuclear leukocyte function in a human periodontal disease.Nature 265, 445–447 (1977).
Degré M.: Viral modulation of phagocytosis and macrophage activities mediated by interferon, p. 37 in FEMSSymp. Bacterial and Viral Inhibition and Modulation of Host Defences, Pisa 1982.
Denman A.M., Pelton B.K., Appleford D., Kinsley M.: Virus infection of lympho-reticular cells and autoimmune diseases.Transplant. Rev. 31, 79–115 (1976).
Faucen N., Desgranges C.: Persistence of human adenovirus 5 in human cord blood lymphoblastoid cell lines transformed by Epstein-Barr virus.Infect.Immun. 29, 1180–1184 (1980).
Friedman H., Specter S.: Immunomodulation by viruses, p. 18 inFEMS Symp. Bacterial and Viral Inhibition and Modulation of Host Defences, Pisa 1982.
Gresser J., Lang J.D.: Relationship between viruses and leukocytes.Prog.Med.Virol. 8, 62–130 (1966).
Hellum K.B., Solberg C.O.: Granulocyte function in bacterial infections in man.Acta Path. Microb.Scand.Sect. C 85, 1–9 (1977).
Holmberg K., Killander J.: Quantitative determination of immunoglobulins (IgG, IgA and IgM) and identification of IgA-type in the gingival fluid.J.Periodont.Res. 6, 1–8 (1971).
Klinkhamer J.M., Zimmerman S.: The function and reliability of the orogranulocytic migratory rate as a measure of oral health.J.Dent.Res. 48, 709–715 (1969).
Larson H.E., Blades R.: Impairment of human polymorphonuclear leukocyte function by influenza virus.Lancet 7954, 283 (1976).
Lehner T.: Immunological aspects of recurrent oral ulcers.Oral Surg. 33, 80–85 (1972).
McDougal W.A.: The effect of topical antigen on the gingiva of sensitized rabbits.J.Periodont. Res. 9, 153–164 (1974).
Mogensen S.C.: Viral interference with the function of phagocytic cells, p. 14–15 in FEMSSymp. Bacterial and Viral Inhibition and Modulation of Host Defences, Pisa 1982.
Nowotny A.:Basic Exercises in Immunochemistry, 2nd ed., p. 272–274. Springer, Berlin-Heidelberg-New York 1979.
Ongrádi J., Kulcsár G., Dán P., Nász I., Horváth J.: Activation of adenovirus type 5 latent infections of tissue culture.Intervirology 14, 310–314 (1980).
Ongrádi J., Sallay K., Kulcsár G., Dán P., Horváth J., Nász I.: Absorption of viruses into oral lymphocytes and decreased antibacterial activity of oral polymorphonuclear leukocytes.Acta Microbiol.Hung. 31, 179–185 (1984).
Ongrádi J., Vámos R., Kulcsár G., Nász I.: Hemmung der Aktivität der Phagocyten mittels Supernatanten der mit Viren infizierten Lymphocytes. (In Hungarian)Kiserl.Orvostud. 37, 199–206 (1985).
Openshaw H., Asher L.V.S., Wohlenberg C., Sekizawa T., Notkins A.L.: Acute and latent infection of sensory ganglia with Herpes simplex virus: Immune control and virus reactivation.J.Gen.Virol. 44, 205–215 (1979).
Pham Ngoc Dinh, Béládi I., Rosztóczy I., Tóth M.: Differential effect of interferon on the production of virus- and mitogen-induced leukocyte inhibitory factors in human leukocyte cultures.Acta Virol. 24, 427–432 (1980).
Rones Y., Hochman N., Ehrlich J., Zakay-Rones Z.: Sensitivity of oral tissues to Herpes simplex virusin vitro.J.Periodont. 54, 91–95 (1983).
Sallay K., Dán P., Geck P., Kulcsár G., Nász I.: Immunofluorescent studies on circulating leukocytes in oral mucosal disorders.Arch.derm.Forsch. 241, 15–21 (1981).
Sallay K., Kulcsár G., Dán P., Nász I., Geck P.: Adenovirus isolation from recurrent oral ulcers.J.Periodont. 44, 712–714 (1973).
Sallay K., Kulcsáe G., Dán P., Nász I., Geck P.: Aetiologie und Prophylaxie der rezidivierenden Aphten.Dtsch.zahnarztl. Z. 30, 570–575 (1975).
Sallay K., Listgaeten M., Sanavi F., Ring I., Nowotny A.: Bacterial invasion of oral tissues of immunosuppressed rats.Infect.Immun. 43, 1091–1094 (1984).
Semenov B.F.: Viruses as nonspecific modulators of immunological reactivity.Acta Virol. 25, 122–128 (1981).
Simon M., Djawari D.: Impairment of some microphage function in recurrent herpes simplex.Acta Dermatovenerol. 62, 434–435 (1982).
Skapski H., Lehner T.: A crevicular washing method for investigating immune components of crevieular fluid in man.J.Periodont.Res. 11, 19–24 (1976).
van der Veen J.,Lambriex M.: Relationship of adenoviruses to lymphocytes in naturally infected human tonsils and adenoids.Infect.Immun. 7, 604–609 (1973).
Wheelock E.F., Toy S.T.: Participation of lymphocytes in viral infections.Adv.Immun. 16, 123–184 (1973).
Wilton J.M., Renggli H.H., Lehner T.: A functional comparison of blood and gingival inflammatory polymorphonuclear leukocytes in man.Clin.Exp.Immun. 27, 152–158 (1977a).
Wilton J.M., Renggli H.H., Lehner T.: The role of Fc and C3b receptors in phagocytosis by inflammatory polymorphonuclear leukocytes in man.Immunology 32, 955–961 (1977b).
Zakay-Rones Z., Ehelich J., Hochman H., Levy R.: The sulcular epithelium as a reservoir for Herpes simplex virus in man.J.Periodont. 44, 779–782 (1973).
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Ongeádi, J., Sallay, K. & Kulcsár, G. The decreased antibacterial activity of oral polymorphonuclear leukocytes coincides with the occurrence of virus-carrying oral lymphocytes and epithelial cells. Folia Microbiol 32, 438–447 (1987). https://doi.org/10.1007/BF02887577
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DOI: https://doi.org/10.1007/BF02887577