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Expression of TGF-β2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract

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Summary

By using RT-PCR and immunohistochemistry, the expressions of transforming growth factorβ 2(TGF-β2) mRNA, proliferating cell nuclear antigen (PCNA) and fibronection (FN) protein in lens epithelial cells (LECs) of age-related nuclear and cortex cataract were detected and compared. The results of RT-PCR revealed that the expression of TGF-β2 mRNA was higher in cortex cataract than in nuclear cataract. Immunohistochemistry demonstrated that the expression of PCNA protein was lower and the expression of FN protein was higher in cortex cataract than in nuclear cataract. It was suggested that TGF-β2, PCNA and FN might take important parts in the process of age-related cataract. Cortex cataract was related to the transdifferentiation of LECs, and nuclear cataract to the proliferation of LECs.

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Authors and Affiliations

  1. Department of Ophthalmology, Zhongnan Hospital, Wuhan University, 430071, Wuhan, China

    Ye Lin, Li Qin, Cai Xiaojun & Deng Ping

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  1. Ye Lin
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  2. Li Qin
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  3. Cai Xiaojun
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  4. Deng Ping
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Additional information

YE Lin, female, born in 1973, M. D., Ph. D.

This project was supported by a grant from Natural Sciences Foundation of Hubei Province (No. 2000J059).

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Lin, Y., Qin, L., Xiaojun, C. et al. Expression of TGF-β2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 25, 578–581 (2005). https://doi.org/10.1007/BF02896023

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  • DOI: https://doi.org/10.1007/BF02896023

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