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Distribution of IgG subclasses after anti-hepatitis B virus immunization with a recombinant vaccine

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International Journal of Clinical and Laboratory Research

Abstract

To assess whether a different IgG subclass distribution was elicited in “low” and “high responders” after vaccination with recombinant hepatitis B virus surface antigen, we selected from 360 vaccine recipients 30 “lowresponder” subjects, with anti-HBs levels of 10–160 mIU/ml, and 40 “high-responder” subjects, with anti-HBs levels greater than 10,000 mIU/ml. In both groups all IgG subclasses were elicited in the anti-HBs response and the greatest contribution was that of IgG 1, followed by IgG2. IgG l was significantly less represented after the second (58%) and third doses (61%) of vaccine in “low responders” compared with “high responders” (65% and 69%). The relative percentage of IgG2 was significantly higher after the second (33%) and third (30%) doses of vaccine in “low responders” than in “high responders” (29% and 26%). In “low responders” the age of vaccine recipients significantly influenced the anti-HBs IgG subclass distribution: IgG2 and IgG4 production was positively correlated with age, whereas the opposite was observed for IgGl. These data support the evidence that: (1) IgGl and IgG2 subclasses are mainly involved in the specific anti-HBs response both in “high” and “low responders”; (2) the relative contribution of specific IgG2 to vaccination is higher in low responders and progressively increases with age.

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Authors and Affiliations

  1. Dipartimento di Clinica Medica e Gastroenterologia, Università di Bologna, Bologna, Italy

    M. C. Honorati & A. Facchini

  2. Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, Via di Barbiano, 1/10, I-40136, Bologna, Italy

    M. C. Honorati, R. M. Borzì, P. Dolzani, S. Toneguzzi & A. Facchini

Authors
  1. M. C. Honorati
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  2. R. M. Borzì
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  3. P. Dolzani
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  4. S. Toneguzzi
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  5. A. Facchini
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Honorati, M.C., Borzì, R.M., Dolzani, P. et al. Distribution of IgG subclasses after anti-hepatitis B virus immunization with a recombinant vaccine. Int J Clin Lab Res 27, 202–206 (1997). https://doi.org/10.1007/BF02912459

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  • DOI: https://doi.org/10.1007/BF02912459

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