Summary
The aim of the investigations was to identify and select the starting material capable of producing differentiated callus and well-growing suspension cultures from immature embryos and inflorescences. The cultivars have been ranked in order of decreasing ability for callus induction on the basis of callus mass. The ability for callus differentiation has been also compared, based upon intensity of the process. Finally, the number of regenerated plants was recorded. All expiants of both immature embryos and inflorescences of the examined cultivars were capable of callus induction. However, the genotypic differences were statistically significant. The greatest mass of callus/embryo was obtained from the cvs. Sakwa, Olcha, Kobra and Almari, and the smallest from the cvs. Korweta and Emika. The greatest mass of callus/inflorescence was obtained from the cvs. Kaja and Zorza. The most intensive callus differentiation was observed from cvs. Almari, Emika, Sakwa and Korweta. Cvs. Tercja and Maltanka regenerated the greatest number of plants per gram of callus. Some tissues were maintained for a long-term culture. The growth index (GI) of callus in successive transfers has also been determined. Suspension cultures from calli both of immature embryo and inflorescence origin of cv. Kaja have been initiated. The growth kinetics of these suspensions was evaluated on the basis of settled and packed cell volume, fresh and dry mass during 17 days of culture. Increase of these parameters was similar for both expiants showing that they are equally good sources of the suspension culture.
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Marcińska, I., Szechyńska, M., Biesaga-Kościelniak, J. et al. Applicability of Polish Winter Wheat (Triticum aestivum L.) Cultivars to Long-Term in vitro Culture. CEREAL RESEARCH COMMUNICATIONS 29, 127–134 (2001). https://doi.org/10.1007/BF03543652
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DOI: https://doi.org/10.1007/BF03543652