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Hyaluronan reversed proteoglycan synthesis inhibited by mechanical stress: possible involvement of antioxidant effect

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Abstract

Introduction

Abnormal mechanical stress loaded on the cartilage leads to the osteoarthritis (OA). Although intraarticular hyaluronan (HA) injection is an effective treatment for OA, the underlying mechanism has not been made clear.

Methods

Mechanical compression was loaded on the bovine cartilage using the Biopress system. Proteoglycan (PG) and reactive oxygen species (ROS) synthesis were measured with [35S] incorporation and fluorescent dye, respectively. Accumulation of peroxynitrite was determined with western blotting using nitrotyrosine antibody.

Results

Mechanical compression inhibited PG synthesis and enhanced ROS. Externally added HA reversed stress-inhibited PG synthesis and attenuated ROS synthesis. HA also significantly decreased the generation of nitrotyrosine.

Conclusions

HA neutralized stress-enhanced ROS synthesis and resulted in the reversing of PG synthesis. These data suggest that HA plays an anabolic effect as an antioxidant.

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Abbreviations

HA:

Hyaluronan

OA:

Osteoarthritis

PG:

Proteoglycan

ROS:

Reactive oxygen species

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Acknowledgments

This work was supported by a research grant from the Scientific Research Fund of the Ministry of Education, Science and Culture of Japan.

Conflict of interest statement

The authors did not receive any outside funding or grants in support of their research for or preparation of this work. Neither they nor a member of their immediate families received payments or other benefits or a commitment or agreement to provide such benefits from a commercial entity.

Author information

Authors and Affiliations

  1. Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan

    Yoshihisa Miki, Takashi Tomiyama, Kanji Fukuda & Chiaki Hamanishi

  2. Department of Rehabilitation Medicine, Kinki University School of Medicine, Osaka, Japan

    Kanji Fukuda

  3. Institute of Advanced Clinical Medicine, Kinki University School of Medicine, Osaka, Japan

    Takeshi Teramura, Yuta Onodera & Kanji Fukuda

  4. Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109-2099, USA

    Toshiki Matsuoka

Authors
  1. Yoshihisa Miki
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  2. Takeshi Teramura
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  3. Takashi Tomiyama
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  4. Yuta Onodera
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  5. Toshiki Matsuoka
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  6. Kanji Fukuda
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  7. Chiaki Hamanishi
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Corresponding author

Correspondence to Kanji Fukuda.

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Responsible Editor: J. Di Battista.

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Miki, Y., Teramura, T., Tomiyama, T. et al. Hyaluronan reversed proteoglycan synthesis inhibited by mechanical stress: possible involvement of antioxidant effect. Inflamm. Res. 59, 471–477 (2010). https://doi.org/10.1007/s00011-009-0147-y

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  • DOI: https://doi.org/10.1007/s00011-009-0147-y

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