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Honokiol: an effective inhibitor of high-glucose-induced upregulation of inflammatory cytokine production in human renal mesangial cells

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Abstract

Objective

To evaluate the regulatory effects of honokiol on high-glucose (HG)-induced inflammatory responses of human renal mesangial cells (HRMCs).

Materials and methods

We performed MTS assays to determine the non-cytotoxic concentration of honokiol for HRMCs. Enzyme-linked immunosorbent assays were performed to analyze the expressions of the proteins interleukin (IL)-1β, IL-18, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1α, RANTES, and prostaglandin (PG) E2. The total nitric oxide (NO) concentration was determined using the Griess reaction.

Results

Treatment with 50 mmol/L glucose markedly increased the level of IL-1β, IL-18, TNF-α, PGE2, NO, TGF-β1, MCP-1, MIP-1α, and RANTES. Honokiol (~20 μmol/L) treatment inhibited the HG-induced expression of inflammatory cytokines such as IL-1β, IL-18, TNF-α, PGE2, NO, and TGF-β1 in a dose-dependent manner. Moreover, it markedly inhibited the expression of chemokines such as MCP-1, MIP-1α, and RANTES, which are upregulated under HG conditions.

Conclusion

Honokiol inhibits the HG-induced expression of inflammatory factors in HRMCs. Honokiol may be considered a promising drug with potent anti-inflammatory activities in addition to its strong anti-cancer, anti-angiogenesis, and anti-neurodegenerative effects.

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Acknowledgments

This work was supported by grants from Science and Technology Foundation (2009C33150, 20090833B24) and Education Foundation (20070114) of Zhejiang Provincial, China. This manuscript is proofread by a native English professional with science background at Elixigen Corporation.

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Authors and Affiliations

  1. Department of Clinical Laboratory, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China

    Jian-Ping Wu & Ying Zhao

  2. Department of Endocrinology, The Affiliated Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

    Wei Zhang & Fang Wu

  3. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Institute of Infectious Diseases, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China

    Lin-Fang Cheng & Hang-Ping Yao

  4. Clinical Medical School, Hangzhou Normal University, Hangzhou, Zhejiang, China

    Jun-Jun Xie

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  1. Jian-Ping Wu
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  2. Wei Zhang
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  3. Fang Wu
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Corresponding author

Correspondence to Hang-Ping Yao.

Additional information

Responsible Editor: Ian Ahnfelt-Rønne.

J.-P. Wu and W. Zhang contributed equally to this work.

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Wu, JP., Zhang, W., Wu, F. et al. Honokiol: an effective inhibitor of high-glucose-induced upregulation of inflammatory cytokine production in human renal mesangial cells. Inflamm. Res. 59, 1073–1079 (2010). https://doi.org/10.1007/s00011-010-0227-z

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  • DOI: https://doi.org/10.1007/s00011-010-0227-z

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