Abstract
A microbial isolate showed strong antibacterial and antifungal activity against various multidrug-resistant test organisms. Based on physiological and biochemical characteristics and 16S ribosomal RNA sequence homology studies, it was found to be similar to Streptomyces capoamus (gene sequence similarity 98%). The antifungal metabolite was produced majorly intracellularly. The active metabolite was extracted and purified by gel filtration chromatography and high-performance liquid chromatography (HPLC). Partial chemical characterization of the active compound has been completed. To the best of our knowledge this strain has not been reported to produce antifungal compounds. The paper presented here describes the activity profile of the strain, classical medium optimization, and purification of the antifungal compound.
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Acknowledgements
The authors would like to thank Mr. D. N. Vishwakarma (STO), Medicinal and Process Chemistry Division, CDRI for HPLC purification and the Council of Scientific and Industrial Research (CSIR), India for financial support.
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Singh, V., Tripathi, C.K.M. & Bihari, V. Production, optimization and purification of an antifungal compound from Streptomyces capoamus MTCC 8123. Med Chem Res 17, 94–102 (2008). https://doi.org/10.1007/s00044-007-9040-9
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DOI: https://doi.org/10.1007/s00044-007-9040-9