Abstract
Profiling the polyphenols in the methanol extract from the bark of Albizia harveyi was performed by HPLC–PDA–ESI–MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin–Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC50 of 3.6 µg/mL and 18.32 mM FeSO4 equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a d-galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (p < 0.05), respectively whereas glutathione was increased by 59.1%. These effects were similar to silymarin which was used as positive control. The extract (100 mg/kg (body weight) mediated a substantial antidiabetic response in streptozotocin-induced diabetic rats manifested by a significant reduction in serum glucose and lipid peroxides and significant increase of serum insulin. Docking of d-(+) catechin and the dimer (epi)catechin-(epi)catechin into the active site of the enzymes human pancreatic α-amylase, maltase-glucoamylase, and aldol reductase revealed that these enzymes may be possible targets via which, the studied Albizia harveyi extract could exert its antidiabetic effect.
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Acknowledgements
The authors would like to thank Dr. C. D. Rubanza (Tanzania Forestry Research Institute, Shinyanga, Tanzania) for plant collection and Dr. B. Wetterauer (IPMB) for his help in collecting LC-MS data.
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Sobeh, M., Mahmoud, M.F., Abdelfattah, M.A.O. et al. Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract. Med Chem Res 26, 3091–3105 (2017). https://doi.org/10.1007/s00044-017-2005-8
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DOI: https://doi.org/10.1007/s00044-017-2005-8