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Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai

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A new broad-spectrum powdery mildew resistance allele Pm2c was identified and mapped in Chinese wheat landrace Niaomai.

Abstract

Chinese wheat landrace Niaomai showed resistance to 27 of 28 Chinese Blumeria graminis f. sp tritici (Bgt) races. Genetic analysis of an F2 population and its derived F2:3 families from the cross Niaomai × Mingxian 169 and backcross population, Niaomai/2*Mingxian 169, indicated that the resistance of Niaomai to Bgt races was conferred by a single dominant resistance gene, temporarily designated PmNM. Molecular tagging showed that PmNM was located on chromosome 5DS and flanked by SSR markers Xcfd81 and Xcfd78 with the genetic distances of 0.1/0.4 cM and 4.9/7.5 cM, respectively. Niaomai showed a different array of responses compared to lines with Pm2a, Pm2b, PmD57-5D, PmLX66, PmX3986-2 and Pm48 genes, sharing the same Xcfd81 allele but differing from Xcfd78 allele for Pm2a and Pm2b lines. Allelism tests based on crosses of Niaomai with Ulka/8*Cc and KM2939 showed that PmNM is allelic to Pm2a and Pm2b. We concluded that PmNM is a new allele of Pm2, re-designated Pm2c. Pm2c could be transferred into wheat cultivars by marker-assisted selection to improve the powdery mildew resistance of breeding cultivars/lines.

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Acknowledgments

We are grateful to Dr. R.A. McIntosh, University of Sydney, Australia, for critically reviewing drafts of this paper, and Professor Hongjie Li, Institute of Crop Science, Chinese Academy of Agricultural Sciences, for comments on the manuscript, and Professor Yilin Zhou, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, for his help with powdery mildew tests. This research was financially supported by the National Key Technology R&D Program (2013BAD01B02), the Chinese Academy of Sciences (#XDA08030107) and the National Natural Science Foundation (31201202 and 31000709).

Conflict of interest

The authors declare that our experiments comply with the current laws of China and we have no conflicts of interest.

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, Hebei, China

    Hongxing Xu, Pengtao Ma, Yanmin Qie, Yunfeng Xu, Xiaotian Zhang & Diaoguo An

  2. School of Bioengineering, Henan University of Technology, Zhengzhou, 450001, Henan, China

    Yanjie Yi

  3. Shijiazhuang Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050041, Hebei, China

    Xiaoyi Fu

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  1. Hongxing Xu
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Correspondence to Diaoguo An.

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Communicated by B. Keller.

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Xu, H., Yi, Y., Ma, P. et al. Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai. Theor Appl Genet 128, 2077–2084 (2015). https://doi.org/10.1007/s00122-015-2568-z

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