Abstract
Key message
A major QTL for SHGD was identified on chromosome 5 with stable expression across environments. The introgression this QTL can overcome the need of colchicine in DH lines development.
Abstract
Genome doubling of haploids is one of the major constraints of large-scale doubled haploid (DH) technology. Improving spontaneous haploid genome doubling (SHGD) is an alternative to overcome this limitation. In this study, we aimed to construct a high-density linkage map based on genotyping by sequencing of single nucleotide polymorphism, to detect QTL and QTL by environment (Q by E) interactions affecting SHGD and to identify the best trait for mapping and selection of haploid male fertility (HMF). To this end, a biparental population of 220 F2:3 families was developed from a cross between A427 (high HMF) and CR1Ht (moderate HMF) to be used as donor. A high-density linkage map was constructed containing 4171 SNP markers distributed over 10 chromosomes with an average distance between adjacent markers of 0.51 cM. QTL mapping for haploid fertile anther emergence, pollen production, tassel size, and HMF, identified 27 QTL across three environments, and Q by E interactions were significant. A major QTL was identified on chromosome 5. This QTL explained over 45% of the observed variance for all traits across all environments. The introgression of this major QTL, using marker-assisted backcrossing, has great potential to overcome the need of using colchicine in DH line development.
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Acknowledgements
This work was supported by USDA’s National Institute of Food and Agriculture (Grant Numbers: IOW04314, IOW01018, IOW05520; IOW05510; NIFA award 2018-51181-28419), the Plant Sciences Institute, Crop Bioengineering Center, R.F. Baker Center for Plant Breeding, K.J. Frey Chair in Agronomy at Iowa State University.
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BT, UKF, and TL design the project and performed the experiments. BT, IGS, JR, SC and TL analyzed the data. BT and IGS wrote the manuscript. All authors read and approved the final manuscript.
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Communicated by Jianbing Yan.
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Trampe, B., dos Santos, I.G., Frei, U.K. et al. QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.). Theor Appl Genet 133, 2131–2140 (2020). https://doi.org/10.1007/s00122-020-03585-1
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DOI: https://doi.org/10.1007/s00122-020-03585-1