Abstract
Aims/hypothesis
In addition to the improvement in insulin sensitivity, it has been shown that thiazolidinediones modulate beta cell function and insulin clearance in type 2 diabetic subjects. However, interactions between all these actions, and confounding factors due to co-morbidities and co-treatments in diabetic individuals, complicate the identification of specific effects. The aim of this pilot study was to investigate the potential acute effects of rosiglitazone on beta cell function and insulin sensitivity by the hyperglycaemic clamp technique in healthy volunteers.
Subjects and methods
Twelve healthy men were included in a randomised, double-blind crossover study. Rosiglitazone (8 mg) or placebo was given orally 45 min before the hyperglycaemic clamp (10 mmol/l for 2 h).
Results
The second phase of the insulin response was significantly decreased by rosiglitazone: 13,066 ± 1,531 vs 16,316 ± 2,813 pmol l−1 110 min in controls (p < 0.05), without change in the first phase. Serum C-peptide was not modified. Rosiglitazone treatment significantly increased insulin clearance (molar ratio of the C-peptide to insulin AUCs: 12.80 ± 1.34 vs 11.38 ± .33, p < 0.05) and the insulin sensitivity index (12.0 ± 1.5 vs 8.5 ± 1.1 μmol m−2 min−1 pmol−1l, p < 0.01).
Conclusions/interpretation
The present results show that a single dose of rosiglitazone rapidly increases insulin clearance and insulin sensitivity index in healthy volunteers, with no direct effect on insulin secretion. The precise mechanisms mediating these actions remain to be determined. ClinicalTrials.gov ID no.: NCT00285142
Introduction
Thiazolidinediones are high-affinity peroxisome proliferator-activated receptor (PPAR)-γ ligands [1]. In addition to their effect on insulin sensitivity, animal studies and long-term clinical studies have reported thiazolidinedione-induced modifications of beta cell function. This action can be either direct, or secondary to the improvement of insulin sensitivity. More recently, rosiglitazone was shown to increase insulin clearance in type 2 diabetic subjects after 12 weeks [2] and 16 weeks [3] of treatment. Although incompletely understood, this effect was considered to be secondary to a decreased liver fat content [3].
In an integrated system such as the human body, and after repeated administration, interactions between all these actions hamper the precise identification of specific effects. To differentiate between direct and secondary effects, evaluating immediate pharmacological actions after a single-dose administration can be helpful. To date, there are no data concerning acute effects of thiazolidinediones in humans. In addition, most evaluations were conducted in diabetic subjects in whom the underlying pathology, possible co-morbidities and co-treatments lead to various confusing factors. Healthy volunteers are a robust model allowing the investigation of pharmacological effects in humans with as few confounding factors as possible. Therefore, the aim of this pilot study was to investigate the potential acute effects of rosiglitazone on beta cell function and insulin sensitivity by the hyperglycaemic clamp technique in healthy volunteers.
Subjects and methods
The protocol, approved by the local ethics committee, was conducted in accordance with the Declaration of Helsinki as revised in 2000 and the Guideline for Good Clinical Practice (ICH Topic E6, 1996).
Subjects
Twelve healthy Europid men, aged 26.5 ± 1.5 years, with normal (21.7 ± 0.4 kg/m2) and stable BMI, normal glycaemia (4.7 ± 0.1 mmol/l) and insulinaemia (31.2 ± 4.6 pmol/l) were included in this study after informed consent.
Study design
This was a single-centre, randomised, double-blind, placebo-controlled crossover study. Participants attended the research centre twice, once for the placebo session and once for the rosiglitazone session, conducted in random order, separated by a 6 week washout period.
Investigations
At 07.00 hours, after an overnight fast at the study centre, a venous catheter was inserted into each forearm, one for administration of 30% dextrose (1.67 mol/l), the other for blood sampling. Rosiglitazone (8 mg) or placebo was administered orally 45 min before the onset of the dextrose infusion. Blood glucose was clamped at 10 mmol/l for 120 min according to DeFronzo et al. [4]. Briefly, participants received a priming dose based on their surface area and initial blood glucose. Continuous dextrose infusion was started 15 min later at a rate adjusted every 5 min on the basis of the prevailing plasma glucose concentrations.
Plasma samples for glucose were taken at −50, −45, −30, −15 and 0 min, then every 5 min after starting the glucose infusion. After centrifugation (6,700 g, 1 min), glucose was immediately analysed (Glucose Analyser 2300 Stat Plus; System Industry, Bourg St Andeol, France) and plasma was frozen at −20°C for later laboratory glycaemia confirmation.
Samples were taken at −50, −45, −30, −15, 0, 2.5, 5, 7.5, 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100 and 120 min for serum insulin and C-peptide and at −45, −30, −15, 0, 15, 20, 30, 45, 60, 90 and 120 min for plasma rosiglitazone. After centrifugation at 2,500 g, serum and plasma were immediately frozen at −20°C.
Immediately after discontinuation of the glucose infusion, urine was collected to determine urinary glucose excretion.
Biochemical analysis
Serum insulin and C-peptide were measured by RIA (BI-INSULIN IRMA and IRMA-C-PEP; CIS Bio-international, Gif-sur-Yvette, France). Plasma glucose was assayed by the glucose oxidase method; rosiglitazone concentrations were assayed by HPLC and tandem mass spectrometric detection (LC-MS/MS; Pharma Bio-Research Group BV, Zuidlaren, the Netherlands).
Calculated indexes
First and second phases of insulin and C-peptide responses were the AUCs of their concentrations from 0 to 10 min and 10 to 120 min, respectively. The insulin sensitivity index was the mean glucose infusion rate divided by the mean plasma insulin concentration from 60 to 120 min, after correcting for urinary glucose excretion [4]. AUCs were determined by the trapezoidal rule.
Fasting glucose and insulin levels were the average of two baseline samples. Insulin clearance was assessed as the molar ratio of the C-peptide to insulin AUCs [5] for each phase of the insulin response and for the total duration of the clamp.
Statistical analysis
Results are expressed as means±SE. Comparisons of means were performed by repeated-measures ANOVA with assessment of carry-over effect, after loge transformation for non-parametric variables. Pearson correlations after logarithmic transformation and Spearman correlations were used when appropriate. Systat 10.0 software for Windows from SPSS (Chicago, IL, USA) was used for statistical analysis. The level of significance was set at p < 0.05.
Results
Mean baseline glycaemia was similar between the treatment and the placebo sessions (5.4 ± 0.1 vs 5.3 ± 0.1 mmol/l, respectively, NS). During the hyperglycaemic clamp, mean glycaemia was identical for the two sessions (10.2 ± 0.1 mmol/l, Fig. 1a). Plasma rosiglitazone concentrations peaked at 875.5 ± 203.1 nmol/l (415 ± 96 μg/l) at 0 min, then decreased and plateaued at about 600 nmol/l (300 μg/l) from 45 min onwards (Fig. 1b).
Figure 1c and d displays the glucose-induced modifications of insulin and C-peptide concentrations. Rosiglitazone did not modify baseline hormone concentrations (insulin: 21.0 ± 2.1 vs 23.4 ± 2.6 pmol/l, NS; C-peptide: 369 ± 36 vs 408 ± 56 pmol/l, NS). Regarding the insulin response, there was no treatment effect on the first phase (AUCinsulin 0–10: 742 ± 121 vs 924 ± 181 pmol l−1 10 min, NS), whereas the second phase was significantly decreased in the presence of rosiglitazone (AUCinsulin 10–120: 13,066 ± 1,531 vs 16,316 ± 2,813 pmol l−1 110 min, p < 0.05). In contrast, serum C-peptide concentrations remained similar between rosiglitazone and placebo sessions, during both the first phase (AUCC-peptide 0–10: 6,699 ± 675 vs 7,285 ± 989 pmol l−1 10 min, NS) and the second phase (AUCC-peptide 10–120: 155,018 ± 12,691 vs 167,962 ± 17,939 pmol l−1 110 min, NS). Accordingly, the insulin clearance was significantly higher after rosiglitazone intake as compared with placebo (Table 1).
The insulin sensitivity index was significantly increased by rosiglitazone (Table 1). The change in insulin sensitivity index (difference between the rosiglitazone and placebo sessions) correlated neither with the changes in insulin AUC (r = 0.196, p = NS) nor with the change in insulin clearance (r = −0.154, p = NS).
No carry-over effect was observed.
Discussion
In this study, we report for the first time acute effects of a thiazolidinedione on both insulin sensitivity and insulin clearance indexes in human subjects after a single oral dose.
The hyperglycaemic clamp technique is considered the gold standard to evaluate beta cell function, and provides an accurate estimation of insulin sensitivity [6]. Our experimental conditions for glycaemia and rosiglitazone concentrations were appropriate. One limitation of our methodology is the estimation of insulin clearance by the ratio AUCC-peptide:AUCinsulin instead of deriving insulin secretion rates by deconvolution of C-peptide concentrations. We preferred this method because given the high individual variability in C-peptide kinetics [7], using fixed standard kinetic parameters in a small sample may be erroneous.
Both animal studies and long-term human studies in type 2 diabetes suggest beneficial effects of thiazolidinediones on pancreatic beta cells, but it remains unclear whether it is a direct effect or secondary to modifications in insulin sensitivity and glucotoxicity. In our acute model, the reduction in insulin concentrations associated with a conserved C-peptide response, suggests an immediate increase in insulin clearance, consistent with the long-term increase previously described [3]. In this latter study, the authors proposed that the rosiglitazone-associated rise in insulin clearance could be secondary to a decreased liver fat content [3]. Here we suggest another mechanism, as the immediate increase of insulin clearance following rosiglitazone intake makes it unlikely to be accounted for by a modification in fat content. Moreover, as the change in insulin clearance is not correlated with the change in the insulin sensitivity index, it is more likely to be a direct effect of rosiglitazone rather than a consequence of the change in insulin sensitivity.
The insulin sensitivity index was immediately increased after acute rosiglitazone intake. One limitation of our methodology is that clamp studies cannot differentiate between an authentic effect on insulin sensitivity or an insulinomimetic effect. Our results challenge the contention that thiazolidinediones act via PPAR-γ-dependent gene expression only, as the rapid onset of the insulin-sensitising action is not compatible with a modulation of gene transcription [8]. Alternatively, a rapid-onset insulin-independent stimulation of glucose uptake was also demonstrated after thiazolidinedione administration in several cell lines. Other explorations are needed to better examine glucose utilisation after acute rosiglitazone administration.
In conclusion, we demonstrated an acute effect of a single dose of rosiglitazone on insulin clearance and insulin sensitivity index in healthy volunteers, suggesting new aspects of thiazolidinedione action in humans. The therapeutic implications of these findings remain to be evaluated.
Abbreviations
- PPAR:
-
peroxisome proliferator-activated receptor
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Acknowledgements
The expert technical assistance of F. Costa, I. Gabillaud and A. Massebiau (research nurses) is gratefully acknowledged. We thank A. Bonardet, B. Bories-Azeau, A. Cadène, J. Foppolo, P. Rambourg and A.-M. Puech-Cathala for their contribution. We also thank S. L. Salli for presubmission editorial assistance. This study was sponsored and financed by the Montpellier University Hospital and received an additional grant from GlaxoSmithKline Laboratory (Marly-le-Roi, France).
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None of the authors has a conflict of interest regarding the content of this paper.
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Farret, A., Chevassus, H., Roux, B. et al. Direct rosiglitazone-induced modifications in insulin secretion, action and clearance: a single-dose hyperglycaemic clamp study. Diabetologia 50, 1384–1387 (2007). https://doi.org/10.1007/s00125-007-0682-4
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DOI: https://doi.org/10.1007/s00125-007-0682-4