Abstract
Aims/hypothesis
Fatty acids of marine origin, i.e. docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) act as hypolipidaemics, but they do not improve glycaemic control in obese and diabetic patients. Thiazolidinediones like rosiglitazone are specific activators of peroxisome proliferator-activated receptor γ, which improve whole-body insulin sensitivity. We hypothesised that a combined treatment with a DHA and EPA concentrate (DHA/EPA) and rosiglitazone would correct, by complementary additive mechanisms, impairments of lipid and glucose homeostasis in obesity.
Methods
Male C57BL/6 mice were fed a corn oil-based high-fat diet. The effects of DHA/EPA (replacing 15% dietary lipids), rosiglitazone (10 mg/kg diet) or a combination of both on body weight, adiposity, metabolic markers and adiponectin in plasma, as well as on liver and muscle gene expression and metabolism were analysed. Euglycaemic–hyperinsulinaemic clamps were used to characterise the changes in insulin sensitivity. The effects of the treatments were also analysed in dietary obese mice with impaired glucose tolerance (IGT).
Results
DHA/EPA and rosiglitazone exerted additive effects in prevention of obesity, adipocyte hypertrophy, low-grade adipose tissue inflammation, dyslipidaemia and insulin resistance, while inducing adiponectin, suppressing hepatic lipogenesis and decreasing muscle ceramide concentration. The improvement in glucose tolerance reflected a synergistic stimulatory effect of the combined treatment on muscle glycogen synthesis and its sensitivity to insulin. The combination treatment also reversed dietary obesity, dyslipidaemia and IGT.
Conclusions/interpretation
DHA/EPA and rosiglitazone can be used as complementary therapies to counteract dyslipidaemia and insulin resistance. The combination treatment may reduce dose requirements and hence the incidence of adverse side effects of thiazolidinedione therapy.
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Introduction
Both dietary and pharmacological interventions are required for therapy of type 2 diabetic patients. Naturally occurring n - 3 long-chain polyunsaturated fatty acids (LC-PUFA), namely eicosapentaenoic acid (EPA; 20:5 n - 3) and docosahexaenoic acid (DHA; 22:6 n - 3), which are abundant in sea fish, act as hypolipidaemics, reduce cardiac events and decrease progression of atherosclerosis, as reviewed by Ruxton et al. [1]. Therefore, n - 3 LC-PUFA are now regarded as healthy constituents of diets for diabetic patients [2, 3]. Several studies in obese humans even demonstrated reductions of adiposity after n - 3 LC-PUFA supplementation [4, 5]. However, n - 3 LC-PUFA appear to have little effect on glycaemic control in diabetic patients [4, 6, 7]. In rodents fed a high-fat diet, n - 3 LC-PUFA efficiently prevented development of obesity [8–10] and of impaired glucose tolerance (IGT) [11, 12].
The effects of n - 3 LC-PUFA are largely mediated by peroxisome proliferator-activated receptors (PPAR), with PPAR-α and PPAR-δ (-β) representing the main targets [13]. However, PPAR-γ, liver X receptor-α, hepatic nuclear factor-4 and sterol regulatory element binding protein-1 are also involved [12, 14, 15]. Besides acting directly as regulatory ligands, n - 3 LC-PUFA also act through their active metabolites, eicosanoids and other lipid molecules [16]. The hypolipidaemic and anti-obesity effects of n - 3 LC-PUFA probably depend on the in situ suppression of lipogenesis and increase of fatty acid oxidation in several tissues [10, 14, 17]. This metabolic switch might reduce accumulation of toxic fatty acid-derivatives, while protecting insulin signalling in liver and muscle [11, 12, 18].
On the other hand, PPAR-γ ligands [19] such as thiazolidinediones (TZDs) are the preferred therapeutic agents for insulin resistance in type 2 diabetic patients. However, TZDs like rosiglitazone and pioglitazone are also associated with unwanted side effects, such as oedema and weight gain [20], possible risk of heart failure [21] and bone loss [22]. These compounds are likely to improve glycaemic control mostly by repartitioning fat away from skeletal muscle [23], while augmenting insulin action in various tissues [24–29].
In spite of the differences in the ability of TZDs and n - 3 LC-PUFA to reverse IGT, both types of compounds reduce adipose tissue inflammation [24, 30], one of the key factors contributing to the development of insulin resistance in obesity, while also increasing adiponectin [12, 15, 24, 25, 31, 32].
We hypothesised that partially overlapping mechanisms of action of n - 3 LC-PUFA and TZDs, namely the activation of metabolic switch by n - 3 LC-PUFA and the induction of lipid repartitioning by TZDs, could have synergistic effects in a combination treatment, leading to an improvement of glycaemic profile so far not described for these strategies when considered separately. We therefore evaluated the effects of: (1) partial replacement of dietary lipids by n - 3 LC-PUFA; (2) a low non obesogenic dose of rosiglitazone; and (3) a combination of both on whole-body parameters of glucose and lipid metabolism in mice fed high-fat diet. To further elucidate the effects of the treatments, a detailed analysis of liver and muscle metabolism was also performed.
Methods
Animals and treatments
Male mice were maintained (two animals per cage) at 22°C on a 12 h light–dark cycle (light on from 06:00 hours) and allowed free access to standard laboratory chow (lipid content ∼3.4% wt/wt; extruded R/M-H diet; Ssniff Spezialdiäten, Soest, Germany). Two types of studies were used.
First, a ‘prevention study’ was performed to characterise the effects of n - 3 LC-PUFA, rosiglitazone and their combination, on developing obesity and IGT in mice fed high-fat diet. At 3 months of age, male C57BL/6N mice (Charles River Laboratories, Sulzfeld, Germany) were randomly assigned to a corn oil-based high-fat diet (cHF; lipid content ∼35.2% wt/wt, mainly corn oil) or to the following treatments: (1) cHF diet supplemented with EPA and DHA (cHF+F) as concentrate of n - 3 LC-PUFA (46% DHA, 14% EPA; 1050TG; EPAX, Lysaker, Norway) replacing 15% of dietary lipids; (2) cHF diet supplemented with rosiglitazone (cHF+TZD) (10 mg/kg diet); and (3) cHF diet supplemented with EPA, DHA and rosiglitazone (cHF+F+TZD) (see Electronic supplementary material [ESM] Tables 1 and 2). Some mice were maintained on the standard chow diet. Various analyses (described below) were performed at 5 to 20 weeks after initiation of treatment. Mice were killed under anaesthesia (09:00–11:00 hours) by cervical dislocation in ad libitum fed state, unless stated otherwise.
Second, in a ‘reversal study’, obesity and IGT were induced by feeding male C57BL/6J mice (The Jackson Laboratory, Bar Harbor, ME, USA) the cHF diet between 3 and 7 months of age, prior to the subsequent 8-week-long treatment as above; i.e. with cHF, cHF+F, cHF+TZD and cHF+F+TZD, respectively.
During treatments, fresh rations of food were distributed daily. Food consumption and body weights were recorded once a week. Experiments were conducted under the guidelines for the use and care of laboratory animals of the Institute of Physiology and followed the ‘Principles of laboratory animal care’ (NIH publication no. 85-23, revised 1985).
Metabolites, hormones and enzymes
Non-esterified fatty acids, triacylglycerols, total cholesterol and insulin were determined in EDTA-plasma [9]. Blood glucose was measured by calibrated glucometers (LifeScan, Milpitas, CA, USA). Total adiponectin was measured by ELISA [31] (R&D Systems, Abingdon, UK). Distribution of adiponectin multimeric complexes was determined using western blotting [33].
Body composition
See ESM for details.
Tissue lipid content
Liver and muscle triacylglycerol content was estimated in ethanolic KOH tissue solubilisates [32]. Ceramide content was estimated in lipid extracts from soleus muscle homogenates using HPLC [34].
Quantitative RT-PCR-based gene expression analysis
Levels of various transcripts were evaluated in total RNA isolated from liver or gastrocnemius muscle [10]. For primers, see ESM Table 3.
Glycogen synthesis in diaphragm
Left and right hemidiaphragms were dissected and separately incubated ex vivo to measure incorporation of glucose into glycogen, as previously described [35] (see ESM).
In vivo hepatic VLDL-triacylglycerol production
The rate of liver triacylglycerol synthesis was evaluated using Triton WR1339 [36] and calculated from the slope of the curve of plasma triacylglycerol levels [37] (see ESM).
Light microscopy and immunohistochemical analysis
Epididymal fat samples were processed to detect MAC-2/galectin and perilipin, using specific antibodies [32, 38] (see ESM).
Glucose tolerance test
Intraperitoneal glucose tolerance test was performed in fasted mice as described [32].
Euglycaemic–hyperinsulinaemic clamp
This was performed as described (ESM) [39–41].
Activation of protein kinase B
Phosphorylation of protein kinase B (Akt/PKB) was measured in soleus muscles using western blots (ESM Fig. 1).
Statistics
Data were analysed by ANOVA and t test, as described in ESM. All values are presented as means ± SE. Comparisons were judged to be significant at p ≤ 0.05.
Results
Prevention of body weight gain and lipid accumulation
Body weight was increased by cHF diet with significant differences between chow and cHF becoming apparent at 4 weeks (Fig. 1a). Treatment by cHF+F or by cHF+TZD diets tended to prevent body weight gain (Fig. 1a). The combination treatment, cHF+F+TZD, reduced body weight gain significantly (Fig. 1a). The same pattern of body weight gain continued throughout the duration of the study, which lasted for 20 weeks. None of the diets affected mean food consumption over the course of the study (Fig. 1b). Body composition was analysed at 15 weeks (Fig. 1c), i.e. when the effect of cHF+F+TZD diet on body weight became significant (Fig. 1a). Protein and lean body mass were similar in mice fed various cHF diets. On the other hand, total weight of eviscerated carcass and total lipid content reflected changes in body weight. Changes in body weight correlated with the changes in adipose tissue mass, with a significant reduction of the content of subcutaneous (not shown) and epididymal (Fig. 1d) fat in the animals treated for 8 weeks by cHF+F+TZD. Histological analysis combined with immunodetection of macrophages revealed cHF diet-induced hypertrophy of adipocytes in epididymal fat at 8 weeks (Fig. 1e), accompanied by increased content of macrophages aggregated in crown-like structures surrounding individual adipocytes (Fig. 1f), indicating induction of low-grade inflammation of adipose tissue in cHF-mice [38]. The induction of adipocyte hypertrophy and macrophage infiltration was significantly counteracted by cHF+F and cHF+TZD diets, with cHF+F+TZD diet exerting the strongest effect.
Indirect calorimetry at 8 weeks did not reveal any effect of the treatments on whole-body oxygen consumption or respiratory exchange ratio in mice with free access to their respective diets. No differences in physical activity between the groups were detected (not shown).
Given the induction of weight gain by rosiglitazone in previous studies on rodents, the induction of a lean phenotype by the cHF+TZD, and especially by the cHF+F+TZD diets was surprising and is, perhaps, related to higher doses of rosiglitazone used in other studies [24, 27]. Indeed, when rosiglitazone content in the diet was increased tenfold (i.e. from 10 to 100 mg rosiglitazone per kg diet), this increase was associated with higher body weight gain as early as 4 weeks after initiation of the feeding (26.3 ± 0.8 g vs 28.5 ± 0.5 g; n = 8; p = 0.04).
Compared with standard chow, the cHF diet induced accumulation of triacylglycerols in liver and gastrocnemius, a mixed-fibre muscle, with a stronger effect observed at 20 than at 8 weeks (Table 1). Compared with cHF, none of the treatments significantly affected the triacylglycerol accumulation, except for cHF+TZD, which increased liver triacylglycerols at 8 weeks. The cHF+F+TZD diet also increased liver triacylglycerol content at 8 weeks, albeit to a smaller extent (Table 1). No differences in the triacylglycerol content between subgroups were observed in soleus, an oxidative muscle, after the prolonged treatment. However, the cHF+F+TZD diet significantly lowered ceramide content in the soleus muscle as compared with cHF-fed mice (Table 1).
Prevention of dyslipidaemia and reduction of hepatic VLDL-triacylglycerol synthesis
In ad libitum fed mice, triacylglycerol levels increased while NEFA and cholesterol levels remained unchanged between 8 and 20 weeks of cHF feeding (Table 2). Triacylglycerol and NEFA levels were significantly suppressed by cHF+F+TZD, even below the levels observed in chow diet-fed mice. Cholesterol levels were reduced at 8 but not at 20 weeks. The other treatments (cHF+F and cHF+TZD) induced smaller or no reduction of plasma lipid levels (Table 2).
We also investigated the effect of 8 weeks of treatment on liver VLDL-triacylglycerol synthesis (Fig. 2). Plasma triacylglycerol levels were measured in fasted mice just prior to and for 3 h after injection of Triton WR-1339. Under these conditions, the nonionic detergent blocks lipolytic degradation of triacylglycerol-rich lipoproteins in the peripheral tissues, with the rise in plasma triacylglycerols proportionate to production of VLDL in the liver. All the treatments significantly decreased the rate of VLDL-triacylglycerol synthesis, with the strongest reduction (approximately twofold) by cHF+F+TZD.
Prevention of IGT and insulin resistance
Insulinaemia increased between 8 and 20 weeks of high-fat feeding, suggesting development of insulin resistance (Table 2). The rise in insulin levels was prevented to a similar extent by cHF+TZD and cHF+F+TZD diets, while the cHF+F diet exhibited a significant but smaller effect.
Both at 8 and 20 weeks, the cHF+F and cHF+TZD diets increased total immunoreactive adiponectin in plasma. An even stronger induction was observed with a combination of the two treatments (Table 2). Multimeric adiponectin complexes in plasma were also analysed. Although the ratio between high molecular weight (HMW) and total adiponectin was similar in the cHF- and chow-fed mice, it was increased by all the other treatments, with the highest additive effect observed in cHF+F+TZD-fed animals, irrespective of treatment duration (Table 2).
To characterise insulin sensitivity and glycaemic control, a glucose tolerance test was performed at 8 weeks (Fig. 3a). Fasted glycaemia was ∼1.4-fold higher in the cHF- as compared with the chow-fed mice and was not significantly affected by any of the treatments (Fig. 3a, b). In contrast, feeding cHF resulted in IGT, as revealed by ∼1.4-fold higher AUC values (glucose tolerance test) in the cHF- compared with the chow-fed mice (Fig. 3c). Both cHF+TZD and cHF+F+TZD diets prevented development of IGT, while cHF+F+TZD displayed the largest improvements in glucose tolerance.
At 8 weeks, a euglycaemic–hyperinsulinaemic clamp was also performed to evaluate precisely the changes in whole-body insulin sensitivity brought about by various treatments (Fig. 3d–i). In the hyperinsulinaemic conditions, the amount of exogenous glucose required to maintain euglycaemia, i.e. the glucose infusion rate, was significantly (∼1.7-fold) higher in mice treated by cHF+F or cHF+TZD than in cHF-fed mice (Fig. 3e), suggesting improvements in insulin sensitivity. Importantly, cHF+F+TZD resulted in a ∼2.4-fold higher glucose infusion rate than that in cHF-fed mice, representing the strongest effect among all the treatments. Hepatic glucose production in the hyperinsulinaemic conditions was decreased in the cHF+F mice to a significantly lower level than in the other subgroups (Fig. 3f), suggesting that DHA/EPA improves hepatic insulin sensitivity. The cHF+F+TZD and cHF+TZD (but not cHF+F) treatments significantly improved whole-body glucose turnover, with cHF+F+TZD showing the most dramatic effect (Fig. 3g). The rate of whole-body glycolysis was not significantly affected by the treatments (Fig. 3h). Whole-body glycogen synthesis was strongly stimulated by cHF+F+TZD, while cHF+F and cHF+TZD diets had no effect (Fig. 3i). No differences in muscle glycogen content between groups were observed (not shown).
Muscle- and liver-specific effects of the treatments
To confirm the results on whole-body glycogen synthesis, measurements were also performed ex vivo, in dissected diaphragms from mice subjected to various treatments (Fig. 4). Diaphragm contains glycolytic and oxidative muscle fibres and is suitable for this type of analysis due to its small diffusion barrier for oxygen [42]. Compared with chow, cHF reduced the rate of basal (∼2.5-fold) and insulin-stimulated (∼2.8-fold) glycogen synthesis. This deleterious effect of cHF feeding was completely prevented by cHF+F+TZD (Fig. 4). Neither cHF+F nor cHF+TZD had any significant effect on basal or insulin-stimulated glycogen synthesis. Improvements in insulin-stimulated glycogen synthesis also correlated with the induction of Akt/PKB phosphorylation at Ser473 in soleus muscle of mice fed cHF+F+TZD (ESM Fig. 1).
Analysis of gene expression from gastrocnemius, a mixed-fibre muscle, revealed a trend (Fig. 5a) for induction by the combination treatment of Pdk4, Cpt1a and Cpt1b, supporting the notion that the combination treatment induced a switch augmenting lipid over glucose catabolism. Interestingly, the cHF+TZD diet strongly induced expression of Scd1, while cHF+F and cHF+F+TZD treatments had an opposite effect.
Analysis of hepatic gene expression (Fig. 5b) revealed a downregulation of gluconeogenic genes Pck2 and G6pc by cHF+F+TZD, while cHF+F and cHF+TZD diets exerted less pronounced effects. The cHF+F+TZD diet strongly induced expression of Acot2, in correlation with upregulation of Cpt1b, suggesting increased turnover of acyl-CoA and fatty acid oxidation in mitochondria. A similar pattern of induction of Acox1, a marker of peroxisomal fatty acid oxidation, was observed. Both cHF+TZD and cHF+F+TZD strongly induced expression of Scd1, while cHF+F had no effect. In contrast, Fas was downregulated by cHF+F and cHF+F+TZD, while cHF+TZD had no effect.
Interestingly, no adverse effects of the combination treatment on enzymatic markers of muscle and liver damage were observed (ESM Table 4).
Reversal of obese phenotype
Given both the high efficacy of the cHF+F+TZD treatment in prevention of the high-fat diet-induced disorders in mice and the failure of n - 3 LC-PUFA to reverse insulin resistance in humans (see Introduction), the effects of cHF+F and cHF+TZD and their combination were also studied in obese mice with IGT (Table 3). Compared with cHF-fed animals, body weight gain, triacylglycerols and NEFA levels were suppressed by cHF+F, while cHF+TZD decreased plasma triacylglycerols and insulin levels. Only cHF+F+TZD affected all the variables studied, showing additive effects of DHA/EPA and rosiglitazone in the reversal of obesity, while decreasing plasma NEFA and cholesterol levels. The cHF+F+ TZD diet also lowered plasma triacylglycerol and insulin concentrations, to a similar extent to that achieved by cHF+TZD (Table 3).
To evaluate the capacity of treatments to reverse IGT in dietary obese mice, a glucose tolerance test was performed 1 week before the end of the study. Fasting glycaemia was decreased to a similar extent by all treatments (Table 3). However, glucose tolerance measured as AUC was markedly improved by cHF+F+TZD, indicating additive effects of DHA/EPA and rosiglitazone in the reversal of IGT. Both cHF+F and cHF+TZD improved glucose tolerance, but to a smaller extent than cHF+F+TZD (Table 3).
Discussion
Combination of life style changes with pharmacological interventions is required for treatment of diabetes and other metabolic diseases associated with obesity. However, safer, more efficacious and less expensive strategies are still needed. We show here that long-term treatment combining partial replacement of dietary lipids by DHA/EPA and a low dose of rosiglitazone markedly and in an additive manner prevented development of dyslipidaemia and insulin resistance, reduced accumulation of body fat and adipocyte hypertrophy, and also induced adiponectin in mice fed a high-fat diet. Importantly, this treatment also reversed IGT in obese mice.
As described before [9, 10], DHA/EPA reduced fat accumulation, without affecting food consumption. Unexpectedly, a relatively low dose of rosiglitazone (10 mg rosiglitazone per kg diet) was sufficient to augment the induction of lean phenotype by DHA/EPA. In contrast, at a tenfold higher dose, rosiglitazone promoted obesity, as observed in most other studies in mice [24, 27]. In humans, rosiglitazone therapy reduced abdominal and increased subcutaneous fat weight [20]. However, weight of both fat depots in mice was decreased by the combination treatment of DHA/EPA with rosiglitazone, suggesting that treatment by a low dose of rosiglitazone combined with dietary n - 3 LC-PUFA intake may also reduce adiposity in humans.
Indirect calorimetry in mice did not reveal any differences in fuel partitioning or energy expenditure between groups. In addition, no difference in physical activity was detected. Therefore, the mechanism for reduced body weight gain in the cHF+F+TZD-treated mice remains unclear. However, even small undetectable changes in the above variables (or in food intake) or their combination, lasting for several weeks, could result in a substantial difference in body weight.
It has been observed previously that treatment by DHA/EPA or rosiglitazone ameliorated adipocyte hypertrophy [9, 43]. In line with these observations, the greatest reduction of adipocyte hypertrophy was observed with the combination of DHA/EPA and rosiglitazone. Compared with large adipocytes, small cells are more insulin-sensitive and less lipolytic, while releasing fewer inflammatory cytokines, as reviewed [20], and more adiponectin [44]. While treatment with DHA/EPA [15, 31] or TZD [15, 25] has previously been shown to induce adiponectin in mice, the current study showed for the first time that the combination of the two increased plasma adiponectin more potently. Importantly, the stimulatory effect of the combination treatment was specific for HMW adiponectin, the form of adiponectin associated with insulin sensitivity [33, 45]. Importantly, the combination treatment also reduced most potently the low-grade adipose tissue inflammation associated with obesity [24, 38]. Thus, the additive effects of DHA/EPA and rosiglitazone in the reduction of fat cell hypertrophy, induction of HMW adiponectin and decrease of adipose tissue inflammation might contribute to the beneficial effects on glucose homeostasis.
In a previous study [24], dual pharmacological activation of PPAR-α (by Wy-14,643) and PPAR-γ (by rosiglitazone) in mice improved insulin sensitivity with increased efficacy compared with treatment by either PPAR agonist alone, while increasing both adiponectin and adiponectin receptors in adipose tissue [24]. In our experiments, the treatments did not significantly affect expression of adiponectin receptor genes Adipor1 and Adipor2, either in muscle or in liver, while DHA/EPA and rosiglitazone still improved insulin sensitivity in an additive manner. Importantly, in our experiments, naturally occurring n - 3 LC-PUFA rather than synthetic PPAR-α agonists were used in the combination with a relatively low dose of rosiglitazone.
Euglycaemic–hyperinsulinaemic clamps in mice and ex vivo measurements in diaphragm muscle showed synergistic induction of glycogen synthesis at the basal and insulin-stimulated conditions by the combination treatment, indicating that skeletal muscle was the major organ responsible for the additive effects of DHA/EPA and rosiglitazone on whole-body glycaemic control. Interestingly, and in accordance with previous studies, neither treatment by DHA/EPA [11, 12, 18], nor rosiglitazone alone (at the relatively low dose used) [23], significantly affected the rate of glycogen synthesis [34]. Increased insulin sensitivity of skeletal muscle is further supported by changes in Akt/PKB phosphorylation. A putative mechanism behind the synergistic effect of the combination treatment is reduction of muscle ceramide concentration. Interestingly, improvements in glucose homeostasis by the treatments became more pronounced with a longer duration of cHF feeding, despite the absence of any effect on triacylglycerol accumulation in the liver and muscle. This observation is not without precedent [12].
In accordance with previous studies, DHA/EPA [11, 12, 18], but not the low dose of rosiglitazone [23], were able to depress hepatic glucose production under hyperinsulinaemic conditions, suggesting improvement of hepatic insulin sensitivity by the former treatment. Given the ability of n - 3 LC-PUFA to prevent development of IGT, the above data also suggest that the effect on hepatic glucose production mentioned above may be required for this protection to occur. On the other hand, the reversal of IGT and insulin resistance by TZDs may be more dependent on the enhancing of insulin action in skeletal muscle. The latter mechanism could be mediated either by TZDs, or even more potently, according to our results, by the combination of DHA/EPA and TZDs.
The additive improvement of glycaemic control correlated with the hypolipidaemic effect of the treatments. Plasma levels of NEFA and triacylglycerols were even lower in mice treated by cHF+F+TZD than in mice fed chow diet, in spite of the higher dietary supply of lipids and the adipose tissue content of the former mice. These results are in accordance with the known hypolipidaemic effects of n - 3 LC-PUFA [1] and rosiglitazone [25] in mice. Interestingly, in humans, only pioglitazone but not rosiglitazone exerted a hypolipidaemic effect [19], documenting that the hypolipidaemic effect of TZDs may be dissociated from their effect on insulin sensitivity [25].
The lowering of NEFA levels in mice subjected to various treatments could reflect several mechanisms, including adipose tissue lipolysis, re-esterification of NEFA in liver and adipose tissue, and stimulation of lipid oxidation in liver [14] and other organs [10] by n - 3 LC-PUFA, as well as by TZDs [26, 27]. The fact that n - 3 LC-PUFAs are able to counteract the inhibition of hepatic fatty acid oxidation by insulin [46] may contribute to the additive effect of the DHA/EPA and rosiglitazone treatment on lipid catabolism. The suppression of plasma triacylglycerol levels possibly resulted from an increased triacylglycerol uptake by muscle and other tissues induced by TZDs [47] and by n - 3 LC-PUFA [48], or from decreased hepatic VLDL-triacylglycerol production. The former mechanism was not studied in our experiments; however, we did demonstrate the inhibition of hepatic VLDL-triacylglycerol production by either DHA/EPA or rosiglitazone, with the strongest effect being observed in the combination treatment. The decrease of hepatic triacylglycerol production by DHA/EPA may represent a functional outcome of the co-ordinated suppression of lipogenic genes by n - 3 LC-PUFA [17]. In addition, stimulation of AMP-activated protein kinase by n - 3 LC-PUFA, resulting in a metabolic switch from lipogenesis to lipid catabolism, may also be involved [49].
In contrast, the mechanism of suppression of VLDL-triacylglycerol formation by rosiglitazone must be different, since rosiglitazone increased Scd1 expression and triacylglycerol content in the liver. The mechanism may reflect an increased rate of fatty acid re-esterification induced by rosiglitazone [50], rather than suppression of de novo lipogenesis in hepatocytes. Our data, in accordance with the effects of the treatments on muscle Scd1, document differential modulation of the genes involved in de novo fatty acid synthesis (Fas) and desaturation (Scd1) by DHA/EPA and rosiglitazone. The induction of SCD1 expression and activity in association with insulin sensitisation by rosiglitazone has been observed before [29], but the underlying mechanism remains unknown.
In conclusion, an original combined treatment using n - 3 LC-PUFA with low-dose rosiglitazone generated additive effects in the prevention as well as reversal of adipose tissue hypertrophy, hyperlipidaemia and impaired glycaemic control in mice fed an obesogenic diet. Multiple mechanisms underlined the beneficial whole-body effects of the combination treatment with a prominent synergistic stimulation of insulin-sensitive muscle glycogen synthesis. The combined use of n - 3 LC-PUFA and TZDs thus represents a potential strategy for treatment of type 2 diabetes and other obesity-associated metabolic disorders. The inclusion of n - 3 PUFA in treatment with TZDs may reduce the dose requirement and the incidence of adverse side effects associated with the TZD-based therapy.
Abbreviations
- Akt/PKB:
-
Protein kinase B
- cHF:
-
Corn oil-based high-fat diet
- cHF+F:
-
cHF diet supplemented with eicosapentaenoic and docosahexaenoic acids
- cHF+F+TZD:
-
cHF diet supplemented with eicosapentaenoic and docosahexaenoic acids and rosiglitazone
- cHF+TZD:
-
cHF diet supplemented with rosiglitazone
- DHA:
-
Docosahexaenoic acid
- EPA:
-
Eicosapentaenoic acid
- HMW:
-
High molecular weight
- IGT:
-
Impaired glucose tolerance
- n - 3 LC-PUFA:
-
n - 3 Long-chain polyunsaturated fatty acids
- PPAR:
-
Peroxisome proliferator-activated receptor
- TZD:
-
Thiazolidinedione
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Acknowledgements
This work was supported by the grants from the Czech Science Foundation (303/08/0664) and the Ministry of Education, Youth and Sports (1M6837805002, COST BMB0602-OC08007) of the Czech Republic. Further support included grants from the European Commission (LSHM-CT-2004-005272, EXGENESIS) to J. Kopecky and V. Mohamed-Ali, and from EPAX AS (Norway), as well as research project AV0Z50110509. The authors thank B. Stankova and E. Tvrzicka for the analysis of fatty acid composition of dietary lipids.
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The authors declare that there is no duality of interest associated with this manuscript.
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O. Kuda and T. Jelenik contributed equally to this work.
An erratum to this article can be found at http://dx.doi.org/10.1007/s00125-009-1388-6
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ESM Text Final (PDF 63.6 KB)
ESM Table 1
Macronutrient composition and energy content of diets, and supplementation by DHA/EPA and rosiglitazone (PDF 11.9 KB)
ESM Table 2
Fatty acid composition of dietary lipids (PDF 37.6 KB)
ESM Table 3
Oligonucleotide primers for quantitative real-time RT-PCR (PDF 12.2 KB)
ESM Table 4
Plasma markers of hepatic and muscle damage (PDF 8.54 KB)
ESM Fig. 1
Phosphorylation of Akt/PKB in soleus muscle. At 5 weeks after initiation of treatment as described, soleus muscles from anaesthetised mice (6 mg pentobarbital sodium per 100 g body weight) were carefully dissected and immediately transferred into pre-warmed 1.5 ml of freshly oxygenated (95% O2, 5% CO2) Krebs–Ringer bicarbonate buffer (pH 7.4), containing 8 mmol/l mannitol and 2 mmol/l pyruvate (30°C). After 10 min, pairs of muscles from each mouse were transferred into fresh medium, in the absence or presence of 2 mU/ml insulin and incubated for 1 h at 30°C. After incubation, muscles were quickly frozen in liquid nitrogen to preserve Akt/PKB phosphorylation. Homogenates from frozen muscles were prepared as described before [4] and total and phosphorylated Akt/PKB were evaluated by western blot analysis using Akt1 mouse antibody (Cell Signaling, Danvers, MA, USA) and Phospho-Akt (Ser473) rabbit antibody (Cell Signaling) on an imaging system (Odyssey Infrared; LI-COR, Bad Homburg, Germany). a Ratio of phosphorylated Akt signal intensity in insulin-stimulated and non-stimulated state. b Representative immunoblot of phosphoAkt (Ser473) and total Akt1. Data are means ± SE; n = 5–7. *p ≤ 0.05 for difference from cHF diet (PDF 436 KB)
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Kuda, O., Jelenik, T., Jilkova, Z. et al. n-3 Fatty acids and rosiglitazone improve insulin sensitivity through additive stimulatory effects on muscle glycogen synthesis in mice fed a high-fat diet. Diabetologia 52, 941–951 (2009). https://doi.org/10.1007/s00125-009-1305-z
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DOI: https://doi.org/10.1007/s00125-009-1305-z