Abstract
Acetylcholinesterase, glutathione-S-transferase and catalase activities were determined in earthworms Eisenia andrei exposed to insecticides (endosulfan, temephos, malathion, pirimiphos-methyl) alone and in a binary combination with the herbicide metolachlor. Metolachlor individually was not acutely toxic, even at high concentrations applied; however, in the treated earthworms metolachlor enhanced the toxicity of endosulfan and temephos by significantly reducing the acetylcholinesterase activity. In binary combination with malathion and pirimiphos-methyl, metolachlor did not increase toxicity. The potentiation character of metolachlor is specific rather than general, and probably depends on the chemical structure of pesticides in the mixture.
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References
Anderson TD, Zhu KY (2004) Synergistic and antagonistic effects of atrazine on the toxicity of organophosphorodithioate and organophosphorothioate insecticides to Chironomus tentans (Diptera: Chironomidae). Pest Biochem Physiol 80:54–64
Belden J, Lydy MY (2000) Impact of atrazine on organophosphate insecticide toxicity. Environ Toxicol Chem 19:2266–2274
Belden JB, Lydy MJ (2001) Effects of atrazine on acetylcholinesterase activity in midges (Chironomus tentans) exposed to organophosphorus insecticides. Chemosphere 44:1685–1689
Berghout A, Buld J, Wenzel E (1990) The cytochrome P450-dependent monooxygenase system of the midgut of the earthworm Lumbricus terrestris. Eur J Pharmacol 183:1885–1886
Bouché MB (1972) Lombriciens de France, Écologie et Systématique, I.N.R.A. Publ Ann Zool Ecol Anim (No. hors-serie) 72:671
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Butler DW, Werrell PA (2005) Inhibitory effect of a herbicide formulation on toxicity of malathion to the worm Eisenia fetida. Bull Environ Contam Toxicol 75:421–428
Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) CRC handbook of methods in oxygen radical research. CRC Press, Boca Raton, pp 283–284
Elhalwagy EA, Zaki NI (2009) Comparative study on pesticide mixture of organophosphorus and pyrethroid in commercial formulation. Environ Toxicol Pharmacol 28:219–224
Ellman GL, Courtney KD, Andreas V, Featherstone RM (1961) A new and rapid colorimetric determination of acetyl cholinesterase activity. Biochem Pharmacol 7:88–95
Gomez-Eyles JL, Svendsen C, Lister L, Martin H, Hodson ME, Spurgeon DJ (2009) Measuring and modelling mixture toxicity of imidacloprid and thiacloprid on Caenorhabditis elegans and Eisenia fetida. Ecotoxicol Environ Saf 72:71–79
Gupta PK (1976) Endosulfan-induced neurotoxicity in rats and mice. Bull Environ Contam Toxicol 15:708–713
Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-S-transferase: the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139
Jin-Clark Y, Lydy MY, Zhu KY (2002) Effects of atrazine and cyanazine on chlorpyrifos toxicity in Chironomus tentans (Diptera: Chironomidae). Environ Toxicol Chem 21:598–603
Lee R (1998) Annelid cytochrome P-450. Comp Biochem Physiol 121C:173–179
Liang J, Zhou Q (2009) Single and binary-combined toxicity of methamidophos, acetochlor and copper acting on earthworms Eisenia foelide. Bull Environ Contam Toxicol 71:1158–1166
Lydy MJ, Austin KR (2004) Toxicity assessment of pesticide mixtures typical of the Sacramento—San Joaquin delta using Chironomus tentans. Arch Environ Contam Toxicol 48:49–55
Lydy MJ, Linck SL (2003) Assessing the impact of triazine herbicides on organophosphate insecticide toxicity to the earthworm Eisenia fetida. Arch Environ Contam Toxicol 45:343–349
Organization for Economic Co-operation and Development (OECD) (1984) Guideline for testing of chemicals No. 207, Earthworm acute toxicity test. Organization for Economic Co-operation and Development, Paris, France
Reinecke SA, Reinecke AJ (2007) The impact of organophosphate pesticides in orchards on earthworms in the Western Cape, South Africa. Ecotoxicol Environ Saf 66:244–251
Ritz C, Streibig JC (2005) Bioassay analysis using R. J Stat Softw 12(5):1–22
Saint-Denis M, Narbonne JF, Arnaud C, Thybaud E, Ribera D (1999) Biochemical responses of the earthworm Eisenia fetida andrei exposed to contaminated artificial soil: effects of benzo(a)pyrene. Soil Biol Biochem 31:1837–1846
Santos MJG, Ferreira V, Soares AMVM, Loureiro S (2011) Evaluation of the combined effects of dimethoate and spirodiclofen on plants and earthworms in a designed microcosm experiment. Appl Soil Ecol 48:294–300
Schreck E, Geret F, Gontier L, Trilhou M (2008) Neurotoxic effect and metabolic responses induced by a mixture of six pesticides on the earthworm Aporrectodea caliginosa nocturna. Chemosphere 71:1832–1839
Xiao N-W, Liu X-H, Li W, Ge F (2006) Effect of herbicide acetochlor on cytochrome P450 monooxygenases and GST of earthworms Eisenia fetida. Chemosphere 18:135–140
Zhou S, Duan C, Michelle WH (2011) Individual and combinated toxic effects of cypermethrin and chlorpyrifos on earthworm. J Environ Sci 23:676–680
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Stepić, S., Hackenberger, B.K., Velki, M. et al. Potentiation Effect of Metolachlor on Toxicity of Organochlorine and Organophosphate Insecticides in Earthworm Eisenia andrei . Bull Environ Contam Toxicol 91, 55–61 (2013). https://doi.org/10.1007/s00128-013-1000-0
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DOI: https://doi.org/10.1007/s00128-013-1000-0