Abstract
In agricultural catchments, aquatic ecosystems can experience a pulse exposure to pesticides. Following such exposure, non-target organisms that are not extirpated may recover. This paper investigates the potential of two duckweed species (Lemna minor and Lemna gibba) to recover from a 7-day exposure to different concentrations (0.4–208 µg L−1) of the herbicide diuron. There was significant inhibition in the growth and biomass after the initial 7-day exposure (e.g. frond number EC50 = 59.2 and 52.2 µg L−1 for L. minor and L. gibba, respectively). Following transfer to clean media, recovery (the highest concentration yielding no significant difference in the effect endpoint from the control) was observed for all effects endpoints at concentrations ranging 60–111 µg L−1 for L. minor and 60–208 µg L−1 for L. gibba. These results suggest that recovery is possible for primary producers at environmentally relevant concentrations considered significant in ecological risk assessment.
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Andrus M, Winter D, Scanlan M, Sullivan S, Bollman W, Waggoner J, Hosmer A, Brain R (2013) Seasonal synchronicity of algal assemblages in three Midwestern streams receiving varying concentrations of atrazine. Sci Total Environ 458–460:125–139
ANZECC (Australian and New Zealand Environment Conservation Council) (2000) An introduction to the Australian and New Zealand Guidelines for fresh and marine water quality. Environment Australia, Canberra
APVMA (Australian Pesticides and Veterinary Medicine Authority) (2011) Diuron: environment assessment. The Australian Government, Canberra
ASTM (American Society for Testing and Materials) (1991) Standard guide 1415-91E: standard guide for conducting static toxicity tests with Lemna gibba G3. Book of ASTM standards. ASTM, Philadelphia, pp 1–10
Bainbridge ZT, Brodie JE, Faithful JW, Sydes DA, Lewis SE (2009) Identifying the land-based sources of suspended sediments, nutrients and pesticides discharged to the Great Barrier Reef from the Tully–Murray Basin, Queensland, Australia. Mar Freshw Res 60:1081–1090
Balakrishnan S, Takeda K, Sakugawa H (2012) Occurrence of diuron and irgarol in seawater, sediments and planktons of Seto Inland Sea, Japan. Geochem J 46:169–177
Baxter L, Brain RA, Prosser RS, Solomon KR, Hanson ML (2013) Sensitivity of green alga to atrazine is not enhanced by previous acute exposure. Environ Pollut 181:325–328
Baxter L, Brain RA, Rodriguez-Gil JL, Hosmer A, Solomon KR, Hanson ML (2014) Response of the green algae Oophila sp., a salamander endosymbiont, to a PSII-inhibitor under laboratory conditions. Environ Toxicol Chem 33:1858–1864
Brain RA, Hosmer AJ, Desjardins D, Kendall TZ, Krueger HO, Wall SB (2012) Recovery of duckweed from time-varying exposure to atrazine. Environ Toxicol Chem 31:1121–1128
Burns M (2011) Catchment-scale ecological risk assessment of pesticides. Dissertation, the University of Sydney, Australia
Canada Environment (2006) Biological test method: test for measuring the inhibition of growth using the freshwater macrophyte, Lemna minor, 2nd edn. Environment Canada, Ottawa
Cedergreen N, Andersen L, Olesen CF, Spliid HH, Streibig JC (2005) Does the effect of herbicide pulse exposure on aquatic plants depend on K-ow or mode of action? Aquat Toxicol 71:261–271
Dorigo U, Berard A, Rimet F, Bouchez A, Montuelle B (2010) In situ assessment of periphyton recovery in a river contaminated by pesticides. Aquat Toxicol 98:396–406
European Food Safety Authority (EFSA) (2005) Conclusion regarding the peer review of the pesticide risk assessment of the active substance Diuron. EFSA scientific report 25, EFSA, Italy
Field JA, Reed RL, Sawyer TE, Griffith SM, Wigington PJ (2003) Diuron occurrence and distribution in soil and surface and ground water associated with grass seed production. J Environ Qual 32:171–179
Greenberg BM, Huang X-D, Dixon DG (1992) Applications of the higher aquatic plant Lemna gibba for ecotoxicological risk assessment. J Aquat Ecosyst Health 1:147–155
Haynes D, Ralph P, Prange J, Dennison B (2000) The impact of the herbicide diuron on photosynthesis in three species of tropical seagrass. Mar Pollut Bull 41:288–293
Izawa S, Good NE (1965) The number of sites sensitive to 3-(3,4-dichlorophenyl)-1,1-dimethylurea, 3-(4-chlorophenyl)-1,1-dimethylurea and 2-chloro-4-(2-propylamino)-6-ethylamino-s-triazine in isolated chloroplasts. BBA Biophys Photosynth 102:20–38
Krieger-Liszkay A, Rutherford AW (1998) Influence of herbicide binding on the redox potential of the quinone acceptor in photosystem-II. Relevance to photodamage and phytotoxicity. Biochem US 37:17339–17344
Lamoree MH, Swart CP, van der Horst A, van Hattum B (2002) Determination of diuron and the antifouling paint biocide Irgarol 1051 in Dutch marinas and coastal waters. J Chromatogr A 970:183–190
McCahon CP, Pascoe D (1990) Episodic pollution: causes, toxicological effects and ecological significance. Funct Ecol 4:375–383
OECD (Organisation for Economic Co-operation and Development) (2006a) Lemna sp., growth inhibition test. OECD Guidelines for the Testing of Chemicals number 221. OECD Publishing, Paris
OECD (Organisation for Economic Co-operation and Development) (2006b) Current approaches in the statistical analysis of ecotoxicity data: a guidance to application. OECD Environment Health and Safety publications series on testing and assessment number 54. OECD Publishing, Paris
Okamura H, Nishida T, Ono Y, Shim WJ (2003) Phytotoxic effects of antifouling compounds on nontarget plant species. Bull Environ Contam Toxicol 71:881–886
Posthuma L, Trass TP, Suter GW II (2002) General introduction to species sensitivity distributions. In: Posthuma L, Suter GW II, Trass TP (eds) Species sensitivity distributions in ecotoxicology, vol 1. Lewis Publishers, Boca Raton, pp 3–17
Prosser RS, Brain RA, Hosmer AJ, Solomon KR, Hanson ML (2013) Assessing the PSII sensitivity and recovery of field-collected periphyton communities under laboratory conditions. Ecotoxicology 22:1367–1383
R Development Core Team (2011) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/
Reinert KH, Giddings JA, Judd L (2002) Effects analysis of time-varying or repeated exposures in aquatic ecological risk assessment of agrochemicals. Environ Toxicol Chem 21:1977–1992
Renger G (1986) Herbicide interaction with photosystem 2: recent developments. Physiol Veg 24:509–521
Ritz C, Streibig JC (2005) Bioassays analysis using R. J Stat Softw 12:1–22
Skark C, Zullei-Seibert N, Willme U, Gatzemann U, Schlett C (2004) Contribution of non-agricultural pesticides to pesticide load in surface water. Pest Manag Sci 60:525–530
Solomon KR, Baker DB, Richards RP, Dixon DR, Klaine SJ, LaPoint TW, Kendall RJ, Weisskopf CP, Giddings JM, Giesy JP, Hall LW, Williams WM (1996) Ecological risk assessment of atrazine in North American surface waters. Environ Toxicol Chem 15:31–74
Teisseire H, Couderchet M, Vernet G (1999) Phytotoxicity of diuron alone and in combination with copper or folpet on duckweed (Lemna minor). Environ Pollut 106:39–45
Vallotton N, Ilda R, Eggen L, Escher BI, Krayenbuhl J, Chevre N (2008) Effect of pulse herbicidal exposure on Scenedesmus vacuolatus: a comparison of two photosystem II inhibitors. Environ Toxicol Chem 27:1399–1407
van Rensen JJS (1982) Molecular mechanisms of herbicide action near photosystem II. Physiol Plant 54:515–521
Zer H, Ohad I (1995) Photoinactivation of photosystem II induces changes in the photochemical reaction center II abolishing the regulatory role of the Qb site in the D1 protein-degradation. Eur J Biochem 231:448–453
Acknowledgments
We acknowledge contributions from researchers of the laboratories of Dr. Keith Solomon and Dr. Paul Sibley at the University of Guelph, Canada to whom we are very grateful. Research was partially funded by the University of Sydney, Australia, Cotton Catchment Communities CRC, Australia and the Cotton Research and Development Corporation, Australia in the form of Ph.D. and travel scholarships to M. Burns, and a Canadian NSERC Discovery Grant to M. Hanson.
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Burns, M., Hanson, M.L., Prosser, R.S. et al. Growth Recovery of Lemna gibba and Lemna minor Following a 7-Day Exposure to the Herbicide Diuron. Bull Environ Contam Toxicol 95, 150–156 (2015). https://doi.org/10.1007/s00128-015-1575-8
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DOI: https://doi.org/10.1007/s00128-015-1575-8