Abstract
A novel bio-analytical method has been devised based on the change in catalytic activity of acetylcholinesterase (AChE) enzyme induced by captan, carbosulfan, 2,3,7,8-tetrachlorodibenzodioxin (TCDD) and pentachlorophenol (PCP) for the investigation of inhibition efficiency and sensitivity using Pt/ZnO/AChE/Chitosan bioelectrode. The inhibition curves of captan, carbosulfan, TCDD and PCP were similar to Michaelis–Menten curve. TCDD held the minimum inhibitor Michaelis–Menten constant (\(K_{M}^{I}\)) value (10.2 nM) in comparison with PCP (10.9 nM), carbosulfan (14.5 nM) and captan (7.9 × 103 nM). The maximum inhibition of AChE enzyme by captan was about 100 %, which was much higher than that of TCDD (72.7 %), PCP (68.1 %) and carbosulfan (47.7 %). The calculated theoretical sensitivity was in the order of TCDD > PCP > carbosulfan > captan. Comparing with TCDD (35.3 %), PCP (47.8 %) and carbosulfan (20.9 %), only the inhibition efficiency of captan (55.0 %) was the maximum. The developed bioelectrode exhibited high recovery and low relative standard deviation in local tap water samples.
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Acknowledgments
The authors are grateful to the Department of Science and Technology, New Delhi, for their financial support [DST/TSG/PT/2008/28, SR/FST/ETI-284/2011(C) and (Nano Mission Council (No. SR/NM/PG-16/2007)]. The research was supported by Professor T. R. Rajagopalan research fund, SASTRA University. We also acknowledge SASTRA University, Thanjavur for extending infrastructural support to carry out the study.
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Nesakumar, N., Ramachandra, B.L., Sethuraman, S. et al. Evaluation of Inhibition Efficiency for the Detection of Captan, 2,3,7,8-Tetrachlorodibenzodioxin, Pentachlorophenol and Carbosulfan in Water: An Electrochemical Approach. Bull Environ Contam Toxicol 96, 217–223 (2016). https://doi.org/10.1007/s00128-015-1705-3
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DOI: https://doi.org/10.1007/s00128-015-1705-3