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Polynucleotide phosphorylase is involved in the control of lipopeptide fengycin production in Bacillus subtilis

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Abstract

Bacillus subtilis is a wealth source of lipopeptide molecules such as iturins, surfactins and fengycins or plipastatins endowed with a range of biological activities. These molecules, designated secondary metabolites, are synthesized via non-ribosomal peptides synthesis (NRPS) machinery and are most often subjected to a complex regulation with involvement of several regulatory factors. To gain novel insights on mechanism regulating fengycin production, we investigated the effect of the fascinating polynucleotide phosphorylase (PNPase), as well as the effect of lipopeptide surfactin. Compared to the wild type, the production of fengycin in the mutant strains B. subtilis BBG235 and BBG236 altered for PNPase has not only decreased to about 70 and 40%, respectively, but also hampered its antifungal activity towards the plant pathogen Botrytis cinerea. On the other hand, mutant strains BBG231 (srfAA−) and BBG232 (srfAC−) displayed different levels of fengycin production. BBG231 had registered an important decrease in fengycin production, comparable to that observed for BBG235 or BBG236. This study permitted to establish that the products of pnpA gene (PNPase), and srfAA− (surfactin synthetase) are involved in fengycin production.

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Fig. 1

[adapted from Schultz et al. 2009; Jacques 2011]

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Acknowledgements

YY was a recipient of PhD scholarship awarded by Campus France through joint French-Iraqi governments program. The authors express their gratitude for “Région des Hauts-de-France” for CPER-FEDER Alibiotech project.

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Authors and Affiliations

  1. Université de Lille, INRA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, F-59000, Lille, France

    Yazen Yaseen, Awa Diop, Frédérique Gancel, Max Béchet, Philippe Jacques & Djamel Drider

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  1. Yazen Yaseen
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  2. Awa Diop
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  3. Frédérique Gancel
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Correspondence to Djamel Drider.

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Communicated by Djamel DRIDER.

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Yaseen, Y., Diop, A., Gancel, F. et al. Polynucleotide phosphorylase is involved in the control of lipopeptide fengycin production in Bacillus subtilis. Arch Microbiol 200, 783–791 (2018). https://doi.org/10.1007/s00203-018-1483-5

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  • DOI: https://doi.org/10.1007/s00203-018-1483-5

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