Abstract
Rice is often infected by bacterial panicle blight disease caused by Burkholderia glumae. Since most studies have assessed the transcriptome of the plant when it is exposed to bacteria, the gene expression of the phytopathogenic bacteria have not been well elaborated during the infection process or in the host cell. Recently, a few researches were conducted to evaluate the in vivo transcriptome of bacteria during the infective process. Most bacterial cells do not express genes involved in pathogenicity in culture medium making it difficult to investigate gene expression of bacterial cells in plant cells. Here, we sought a simulated patho-system that would allow bacterial cells to express their pathogenic genes. Thus, rice root exudates (RE) and bacterial N-acyl homoserine lactone (AHL) were used and their effects on bacterial gene expression were assessed. Transcription patterns of B. glumae virulence determinants showed that enrichment medium (LB + RE + C8-HSL) could significantly induce virulence factor genes compared with Luria Bertani (LB; control) medium. The data indicate that the artificial environment is similar to the real patho-system, and that this induced maximum relevant gene expression. In this model system, bacterial gene expression changes are traceable in the infection process. Bacterial cells exposed to either an artificial environment or LB + RE + C8-HSL behaved similarly to the natural environment in situ.
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Acknowledgements
This research study was funded by the following: the Innovation Project of Science and Technology of China Agricultural Academy of Science (CAAS-ASTIP-2013-CNRRI); the National Key Research and Development Program of China (2016YFD0200801); the Special Fund of Central Authorities Commonwealth Scientific Research Institute Fundamental Operating Expense (2012ZL096); and the National Natural Science Foundation of China (31601288).
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AM designed and performed the experiments, analyzed and interpreted the data, wrote the original draft manuscript. BRG helped in data interpretation, edited and revised the manuscript. YH provided materials and contributed in analyzing the data. SH designed the experiments, interpreted the data, reviewed the article, directed the project and acted as corresponding author. All authors read and approved the final manuscript.
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Communicated by Djamel Drider.
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Mirghasempour, A., Glick, B.R., Hou, Y. et al. A system to study the expression of phytopathogenic genes encoded by Burkholderia glumae. Arch Microbiol 203, 383–387 (2021). https://doi.org/10.1007/s00203-020-01986-w
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DOI: https://doi.org/10.1007/s00203-020-01986-w