Abstract
A yellow-orange, rod-shaped (0.3–0.4 × 2.2–3.8 µm), Gram-stain-negative, non-motile, atrichous bacterium, designated strain 1062T, was isolated from tidal zone sediment collected from Xiaoshi Island, Weihai, Shandong Province, China. Growth of strain 1062T occurred at 15–43 °C (optimum, 37 °C), pH 6.0–9.5 (optimum, pH 7.5) and with 0.5–7.0% (w/v) NaCl (optimum, 2.0–3.0%). Nitrate was not reduced to nitrite or nitrogen. Positive for catalase and oxidase activity, as well as the hydrolysis of casein, DNA, starch and Tweens 20, 40, 60, but negative for hydrolysis of alginate, CM-cellulose, agar and Tweens 80. The 16S rRNA gene sequence comparisons indicated that strain 1062T had a sequence similarity of 96.2% with Fulvivirga lutimaris KCTC 42720T and 94.3% with Fulvivirga kasyanovii KCTC 12832T. Phylogenetic analysis showed that strain 1062T belonged to the genus Fulvivirga. The genomic DNA G + C content was 45.1 mol%. The ANI values of strain 1062T in comparison to F. kasyanovii KCTC 12832T and F. lutimaris KCTC 42720T were all lower than 70%. The dDDH values of strain 1062T in comparison to F. kasyanovii KCTC 12832T and F. lutimaris KCTC 42720T were all lower than 20%. The average amino acid identity values of strain 1062T in comparison to F. kasyanovii KCTC 12832T and F. lutimaris KCTC 42720T were all lower than 60%. The major fatty acids (> 5%) of strain 1062T were iso-C15:0, iso-C15:0 G, iso-C15:0 3OH, iso-C17:0 3-OH and summed feature 3 (C16:1ω7c/C16:1ω6c). The polar lipids consist of an aminolipid, phosphatidylethanolamine, phosphatidyglycerol and diphosphatidylglycerol. The major respiratory quinone was MK-7. Based on the results of physiological, biochemical and chemotaxonomic characteristics, the strain 1062T (= KCTC 72868T = MCCC 1H00499T) was identified as a new species of the genus Fulvivirga and the name Fulvivirga sedimenti sp. nov, is proposed.
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Abbreviations
- KCTC:
-
Korean Collection for Type Culture
- MCCC:
-
Marine Culture Collection of China
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- dDDH:
-
Digital DNA–DNA hybridization
- AAI:
-
Average amino acid identity
- ANI:
-
Average nucleotide identity
- MEGA:
-
Molecular Evolutionary Genetics Analysis
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Acknowledgements
The implementation of scanning electron microscope was supported by the Physical-Chemical Materials Analytical and Testing Center of Shandong University at Weihai.
Funding
This work was supported by Science & Technology Fundamental Resources Investigation Program (Grant No. 2019FY100700), National Natural Science Foundation of China (32200003) and Shandong University (Weihai) interdisciplinary cultivation project “Research on resources & environment and high-quality development of marine economy”.
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XL and HY isolated the strain 1062T. Shao-Juan Du helped process cell samples and photograph cell morphology. XL and HY finished the experiment and manuscript. ZD and MY offered experiment guidance and critical revision of manuscripts. All authors contributed to the article and approved the submitted version.
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Communicated by Erko Stackebrandt.
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The GenBank accession number for the 16S rRNA gene sequence of strain 1062T is MW391774. The whole-genome shotgun project of strain 1062T has been deposited into DDBJ/EMBL/GenBank under the accession number JAIXNE000000000.
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Liu, Xj., Yu, H., Du, SJ. et al. Fulvivirga sedimenti sp.nov, isolated from the sediment of oceanic tidal zone. Arch Microbiol 204, 727 (2022). https://doi.org/10.1007/s00203-022-03344-4
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DOI: https://doi.org/10.1007/s00203-022-03344-4