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Individual cell migration analysis using fiber-optic bundles

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Abstract

In this paper we describe a novel optical fiber-based technology for analyzing cell migration. Cells were labeled with a membrane-bound fluorescent dye and distributed onto a polished optical fiber bundle. When a cell passes over one of the individual fibers in the bundle, the membrane-bound dye causes a large intensity increase, which stays for a given “residence time” until the cell departs from the fiber. Residence time increases significantly upon exposure to an antimigratory drug, indicating a decrease in cell migration. This approach provides a simple migration assay and does not require sophisticated tracking software. By using optical fiber bundles containing smaller individual fibers with higher spatial resolution, this approach was employed to develop a migration assay based on subcellular imaging. The subcellular imaging platform allows for rapid analysis of migratory potential, reducing experimental time from several hours in a standard assay to 5 min using this technology.

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Acknowledgments

This initial work was supported by a grant from the National Institutes of Health, Institute of General Medical Sciences.

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Authors and Affiliations

  1. Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, MA, 02155, USA

    Christopher DiCesare, Israel Biran & David R. Walt

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  1. Christopher DiCesare
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  2. Israel Biran
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  3. David R. Walt
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Corresponding author

Correspondence to David R. Walt.

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DiCesare, C., Biran, I. & Walt, D.R. Individual cell migration analysis using fiber-optic bundles. Anal Bioanal Chem 382, 37–43 (2005). https://doi.org/10.1007/s00216-005-3142-3

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  • DOI: https://doi.org/10.1007/s00216-005-3142-3

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