Abstract
We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.
Similar content being viewed by others
References
Sperling RA, Rivera GP, Zhang F, Zanella M, Parak WJ (2008) Chem Soc Rev 37:1896–1908
Myroshnychenko V, Rodriguez-Fernandez J, Pastoriza-Santos I, Funston AM, Novo C, Mulvaney P, Liz-Marzan LM, de Abajo FJG (2008) Chem Soc Rev 37:1792–1805
Xia F, Zuo XL, Yang RQ, Xiao Y, Kang D, Vallee-Belisle A, Gong X, Yuen JD, Hsu BBY, Heeger AJ, Plaxco KW (2010) Proc Natl Acad Sci USA 107:10837–10841
Storhoff JJ, Elghanian R, Mucic RC, Mirkin CA, Letsinger RL (1998) J Am Chem Soc 120:1959–1964
Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA (1997) Science 277:1078–1081
Wang H, Wang YX, Jin JY, Yang RH (2008) Anal Chem 80:9021–9028
Tan YN, Su XD, Liu ET, Thomsen JS (2010) Anal Chem 82:2759–2765
Wang XY, Zou MJ, Xu X, Lei R, Li KA, Li N (2009) Anal Bioanal Chem 395:2397–2403
Zhang JQ, Wang YS, He Y, Jiang T, Yang HM, Tan X, Kang RH, Yuan YK, Shi LF (2010) Anal Biochem 397:212–217
Chiappelli F, Iribarren FJ, Prolo P (2006) Bioinformation 1:331–334
Perera S, Uddin M, Hayes JA (1997) Int J Behav Med 4:170–178
Levinson SS, Elin RJ, Yam L (2002) Clin Chem 48:1131–1132
Morsky P, Aine E (1983) Clin Chim Acta 129:201–209
Gupta DK, Vonfigura K, Hasilik A (1987) Clin Chim Acta 165:73–82
Virella G (1977) Clin Chim Acta 75:107–115
Schneider N, Weigel I, Werkmeister K, Pischetsrieder M (2010) J Agric Food Chem 58:76–81
Gao P, John MR, Schmidtgayk H, Arndt B, Scheida M, Theuer D (1995) Clin Chim Acta 239:167–177
Rauch P, Poplstein M, Hochel I, Fukal L, Ferri E, Abagnato CA, Girotti S, Roda A (1995) J Biolumin Chemilumin 10:35–40
Sato R, Takeyama H, Tanaka T, Matsunaga T (2001) Appl Biochem Biotechnol 91–3:109–116
Tombelli S, Minunni A, Mascini A (2005) Biosens Bioelectron 20:2424–2434
Cox JC, Ellington AD (2001) Bioorg Med Chem 9:2525–2531
Cho EJ, Collett JR, Szafranska AE, Ellington AD (2006) Anal Chim Acta 564:82–90
Wang J, Liu B (2009) Chem Commun 2284–2286
Cheng AKH, Ge B, Yu HZ (2007) Anal Chem 79:5158–5164
Peng YG, Zhang DD, Li Y, Qi HL, Gao Q, Zhang CX (2009) Biosens Bioelectron 25:94–99
Wang B, Yu C (2010) Angew Chem Int Edit 49:1485–1488
Huang HP, Jie GF, Cui RJ, Zhu JJ (2009) Electrochem Commun 11:816–818
Tran DT, Janssen KPF, Pollet J, Lammertyn E, Anne J, Van Schepdael A, Lammertyn J (2010) Molecules 15:1127–1140
Wang XY, Xu Y, Xu XA, Hu K, Xiang MH, Li LM, Liu F, Li N (2010) Talanta 82:693–697
Li HX, Rothberg L (2004) Proc Natl Acad Sci USA 101:14036–14039
Wang LH, Liu XF, Hu XF, Song SP, Fan CH (2006) Chem Commun 36:3780–3782
Li HX, Rothberg LJ (2004) J Am Chem Soc 126:10958–10961
Xu XHN, Huang S, Brownlow W, Salaita K, Jeffers RB (2004) J Phys Chem B 108:15543–15551
Lee MH, Chen YC, Ho MH, Lin HY (2010) Anal Bioanal Chem 397:1457–1466
Wang YY, Pu KY, Liu B (2010) Langmuir 26:10025–10030
Acknowledgment
This work was supported by the National Natural Science Foundation of China (nos. 20975004 and 21035005) and Instrumental Analysis Fund of Peking University.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary materials
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Wang, X., Xu, Y., Chen, Y. et al. The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme. Anal Bioanal Chem 400, 2085–2091 (2011). https://doi.org/10.1007/s00216-011-4943-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-011-4943-1