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Chemiluminescence detection flow cells for flow injection analysis and high-performance liquid chromatography

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Abstract

We have examined a range of new and previously described flow cells for chemiluminescence detection. The reactions of acidic potassium permanganate with morphine and amoxicillin were used as model systems representing the many fast chemiluminescence reactions between oxidising agents and organic analytes, and the preliminary partial reduction of the reagent was exploited to further increase the rates of reaction. The comparison was then extended to high-performance liquid chromatography separations of α- and β-adrenergic agonists, with permanganate chemiluminescence detection. Flow cells constructed by machining novel channel designs into white polymer materials (sealed with transparent films or plates) have enabled improvements in mixing efficiency and overall transmission of light to the photodetector.

Chemiluminescence from the reaction of morphine and permanganate in a serpentine flow cell.

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Acknowledgements

The authors thank the Faculty of Science and Technology (Deakin University) and the Australian Research Council (Future Fellowship FT100100646) for funding this research; Geoffrey P. McDermott and Kerryn F. Saltmarsh (School of Life and Environmental Sciences, Deakin University) for assistance with the HPLC experiments; and Donna Squire (Knowledge Media Division, Deakin University) for the photography. J.M.T acknowledges the receipt of an Australian Postgraduate Award.

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Authors and Affiliations

  1. School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, 3216, Australia

    Jessica M. Terry, Neil W. Barnett & Paul S. Francis

  2. School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M60 1QD, UK

    Stephan Mohr, Peter R. Fielden & Nick J. Goddard

  3. Global FIA, P.O. Box 480, Fox Island, WA, 98333, USA

    Don C. Olson & Duane K. Wolcott

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  1. Jessica M. Terry
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  2. Stephan Mohr
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  8. Paul S. Francis
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Correspondence to Paul S. Francis.

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Published in the special issue Young Investigators in Analytical and Bioanalytical Science with guest editors S. Daunert, J. Bettmer, T. Hasegawa, Q. Wang and Y. Wei.

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Terry, J.M., Mohr, S., Fielden, P.R. et al. Chemiluminescence detection flow cells for flow injection analysis and high-performance liquid chromatography. Anal Bioanal Chem 403, 2353–2360 (2012). https://doi.org/10.1007/s00216-012-5902-1

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