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On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes

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Abstract

In order to reveal the time-depending mercury species uptake by human astrocytes, a novel approach for total mercury analysis is presented, which uses an accelerated sample introduction system combined on-line with an inductively coupled plasma mass spectrometer equipped with a collision/reaction cell. Human astrocyte samples were incubated with inorganic mercury (HgCl2), methylmercury chloride (MeHgCl), and thimerosal. After 1-h incubation with Hg2+, cellular concentrations of 3 μM were obtained, whereas for organic species, concentrations of 14–18 μM could be found. After 24 h, a cellular accumulation factor of 0.3 was observed for the cells incubated with Hg2+, whereas the organic species both showed values of about 5. Due to the obtained steady-state signals, reliable results with relative standard deviations of well below 5 % and limits of detection in the concentration range of 1 ng L−1 were obtained using external calibration and species-unspecific isotope dilution analysis approaches. The results were further validated using atomic fluorescence spectrometry.

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Acknowledgments

The authors would like to thank Hans-Joachim Galla from the Institute of Biochemistry, University of Münster for providing the human astrocyte cell line.

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Authors and Affiliations

  1. Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 28/30, 48149, Münster, Germany

    Christoph A. Wehe, Michael Holtkamp, Georgina M. Thyssen, Michael Sperling & Uwe Karst

  2. Institute of Food Chemistry, University of Münster, Corrensstr. 45, 48149, Münster, Germany

    Imke Pieper & Tanja Schwerdtle

  3. European Virtual Institute for Speciation Analysis (EVISA), Mendelstr. 11, 48149, Münster, Germany

    Michael Sperling

  4. Institute of Nutritional Sciences, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany

    Tanja Schwerdtle

Authors
  1. Christoph A. Wehe
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  2. Imke Pieper
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  3. Michael Holtkamp
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  4. Georgina M. Thyssen
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  5. Michael Sperling
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  6. Tanja Schwerdtle
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  7. Uwe Karst
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Correspondence to Uwe Karst.

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Wehe, C.A., Pieper, I., Holtkamp, M. et al. On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes. Anal Bioanal Chem 406, 1909–1916 (2014). https://doi.org/10.1007/s00216-013-7608-4

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  • DOI: https://doi.org/10.1007/s00216-013-7608-4

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