Abstract
The MTB-DR-RIF 9G membrane can detect by detecting multiple mutations in multiple codons. The MTB-DR-RIF 9G membrane possesses clinical applicability in point-of-care settings for the following reasons: (i) 100 % similar results with that of the sequencing analysis for clinical samples, (ii) discrimination of the multiple mutations in multiple codons, (iii) a specific/non-specific hybridization ratio higher than 350:1, and (iv) the sensitivity was found to be 1–10 copies/test for detection and discrimination of the wild and mutant TB strains.
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This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Project No.: HI13C0858).
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The authors declare that they have no competing interests.
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Danishmalik Rafiq Sayyed and Satish Balasaheb Nimse contributed equally to this work.
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Sayyed, D.R., Nimse, S.B., Song, KS. et al. MTB-DR-RIF 9G membrane: a platform for multiplex SNP detection of multidrug-resistant TB. Anal Bioanal Chem 407, 5739–5745 (2015). https://doi.org/10.1007/s00216-015-8754-7
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DOI: https://doi.org/10.1007/s00216-015-8754-7