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Development and optimization of a novel sample preparation method cored on functionalized nanofibers mat-solid-phase extraction for the simultaneous efficient extraction of illegal anionic and cationic dyes in foods

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Abstract

A simple and efficient three-step sample preparation method was developed and optimized for the simultaneous analysis of illegal anionic and cationic dyes (acid orange 7, metanil yellow, auramine-O, and chrysoidine) in food samples. A novel solid-phase extraction (SPE) procedure based on nanofibers mat (NFsM) was proposed after solvent extraction and freeze-salting out purification. The preferred SPE sorbent was selected from five functionalized NFsMs by orthogonal experimental design, and the optimization of SPE parameters was achieved through response surface methodology (RSM) based on the Box-Behnken design (BBD). Under the optimal conditions, the target analytes could be completely adsorbed by polypyrrole-functionalized polyacrylonitrile NFsM (PPy/PAN NFsM), and the eluent was directly analyzed by high-performance liquid chromatography-diode array detection (HPLC-DAD). The limits of detection (LODs) were between 0.002 and 0.01 mg kg−1, and satisfactory linearity with correlation coefficients (R > 0.99) for each dye in all samples was achieved. Compared with the Chinese standard method and the published methods, the proposed method was simplified greatly with much lower requirement of sorbent (5.0 mg) and organic solvent (2.8 mL) and higher sample preparation speed (10 min/sample), while higher recovery (83.6–116.5%) and precision (RSDs < 7.1%) were obtained. With this developed method, we have successfully detected illegal ionic dyes in three common representative foods: yellow croaker, soybean products, and chili seasonings.

Schematic representation of the process of the three-step sample preparation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Project No. 81473019).

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Author notes

  1. Feifei Qi and Ningge Jian contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, China

    Feifei Qi, Ningge Jian, Liangliang Qian, Weixin Cao & Qian Xu

  2. Suzhou Municipal Center for Disease Prevention and Control, Suzhou, Jiangsu, 215004, China

    Jian Li

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  1. Feifei Qi
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Correspondence to Qian Xu or Jian Li.

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Qi, F., Jian, N., Qian, L. et al. Development and optimization of a novel sample preparation method cored on functionalized nanofibers mat-solid-phase extraction for the simultaneous efficient extraction of illegal anionic and cationic dyes in foods. Anal Bioanal Chem 409, 5697–5709 (2017). https://doi.org/10.1007/s00216-017-0510-8

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  • DOI: https://doi.org/10.1007/s00216-017-0510-8

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