Abstract
Smartphone biosensors could be cost-effective, portable instruments to be used for the readout of liquid colorimetric assays. However, current reported smartphone colorimetric readers have relied on photos of liquid assays captured using a camera, and then analyzed using software programs. This approach results in a relatively low accuracy and low generality. In this work, we reported a novel smartphone colorimetric reader that has been integrated with an ambient light sensor and a 3D printed attachment for the readout of liquid colorimetric assays. The portable and low-cost ($0.15) reader utilized a simplified electronic and light path design. Furthermore, our reported smartphone colorimetric reader can be compatible with different smartphones. As a proof of principle, the utility of this device was demonstrated using it in conjunction with an enzyme-linked immunosorbent assay to detect zearalenone. Results were consistent with those obtained using a professional microplate reader. The developed smartphone colorimetric reader was capable of providing scalable, cost-effective, and accurate results for liquid colorimetric assays that related to clinical diagnoses, environment pollution, and food testing.
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Acknowledgements
This work was supported by a grant from the National Key Development Program of China (2016YFD0500600); National Key Technology R & D Program, No. 2008BAK42B-05; and Guangdong Province Key Scientific Research, No. 2013A022100031.
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Chen, Y., Fu, Q., Li, D. et al. A smartphone colorimetric reader integrated with an ambient light sensor and a 3D printed attachment for on-site detection of zearalenone. Anal Bioanal Chem 409, 6567–6574 (2017). https://doi.org/10.1007/s00216-017-0605-2
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DOI: https://doi.org/10.1007/s00216-017-0605-2