Abstract
The objective of this study was to investigate the effect of long-term frozen storage (2 years) of pork on colour stability, lipid and protein oxidation, drip/thawing losses and intramuscular fatty acid composition, as well as to examine the relationships among them. Pork quality parameters were significantly altered by frozen storage during 2 years, causing an increase in pH values, drip and thawing losses and metmyoglobin percentage, and a decrease in colour indices (a* and b* values) and oxymyoglobin percentage. The percentage of intramuscular fat and total saturated and monounsaturated fatty acids were significantly higher in pork after 2 years of storage at −20 °C, whereas it had the lowest values of polyunsaturated fatty acids, especially n-3 long chain fatty acids. Furthermore, the relationships among colour stability, lipid and protein oxidation, thawing losses and fatty acid profile revealed the complex interrelations generated during frozen storage.
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References
Leygonie C, Britz TJ, Hoffman LC (2012) Impact of freezing and thawing on the quality of meat: review. Meat Sci 91:93–98
Hansen E, Juncher D, Henckel P, Karlsson A, Bertelsen G, Skibsted LH (2004) Oxidative stability of chilled pork chops following long term freeze storage. Meat Sci 68:479–484
Jeong JY, Kim GD, Yang HS, Joo ST (2011) Effect of freeze–thaw cycles on physicochemical properties and colour stability of beef semimembranosus muscle. Food Res Int 44:3222–3228
Jeremiah LE (1996) Freezing effects on food quality. CRC Press, New York
Decker EA, Xiong YL, Calvert JT, Crum AD, Blanchart SP (1993) Chemical, physical and functional properties of oxidized turkey white muscle myofibrillar proteins. J Agric Food Chem 41:186–189
Berlett BS, Stadtman ER (1997) Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 272:20313–20316
Stadman ER, Levine RL (2000) Protein oxidation. Ann N Y Acad Sci 899:191–208
Hernández P, Navarro J, Toldrá F (1999) Effect of frozen storage on lipids and lipolytic activities in the Longissimus dorsi muscle of the pig. Eur Food Res Technol 208:110–115
Owen JE, Lawrie RA (1975) The effect of an artificially induced high pH on the susceptibility of minced porcine muscle to undergo oxidative rancidity under frozen storage. Int J Food Sci 10:169–180
Faustman C, Sun Q, Mancini R, Suman SP (2010) Review: myoglobin and lipid oxidation interactions: mechanistic bases and control. Meat Sci 86:86–94
Añón MC, Calvelo A (1980) Freezing rate effects on the drip loss of frozen beef. Meat Sci 4:1–14
Estévez M, Ventanas S, Heinonen M, Puolanne E (2011) Protein carbonylation and water-holding capacity of pork subjected to frozen storage: effect of muscle type, premincing, and packaging. J Agric Food Chem 59:5435–5443
Bertram HC, Andersen RH, Andersen HJ (2007) Development in myofibrillar water distribution of two pork qualities during 10-month freezer storage. Meat Sci 75:128–133
Boletín Oficial del Estado (BOE) (2007) Ley Española 32/2007 sobre el cuidado de los animales, en su explotación, transporte, experimentación y sacrificio. BOE 268:45914–45920
Alonso V, Najes LM, Provincial L, Guillén E, Gil M, Roncalés P, Beltrán JA (2012) Influence of dietary fat on pork eating quality. Meat Sci 92:366–373
Alonso V, Tenas J, Muela E, Roncalés P, Beltrán JA (2013) Influence of frozen long-storage duration on pork quality. Proc 59th Int Congr Meat Sci Technol S5:64–68
Krzywicki K (1979) Assessment of relative content of myoglobin, oxymyoglobin and metmyoglobin at the surface of beef. Meat Sci 3:1–10
Tsuruga T, Ito T, Kanda M, Niwa S, Kitazaki T, Okugawa T, Hatao S (1994) Analysis of meat pigments with tissue spectrophotometer TS-200. Meat Sci 36:423–434
Honikel KO (1998) Reference methods for the assessment of physical characteristics of meat. Meat Sci 49:447–457
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Psychol 37:911–914
Carrilho MC, López M, Campo MM (2009) Effect of the fattening diet on the development of the fatty acid profile in rabbits from weaning. Meat Sci 82:37–43
Pfalzgraf A, Frigg M, Steinhart H (1995) Alpha-tocopherol contents and lipid oxidation in pork muscle and adipose tissue during storage. J Agric Food Chem 43:1339–1342
Ventanas S, Estevez M, Tejeda JF, Ruiz J (2006) Protein and lipid oxidation in Longissimus dorsi and dry cured loin from Iberian pigs as affected by crossbreeding and diet. Meat Sci 72:647–655
IBM SPSS (2010). Statistical package for social sciences for Windows (version 19.0). SPSS Inc, Chicago, IL
Brewer MS, Harbers CAZ (1991) Effect of packaging on physical and sensory characteristics of ground pork in long-term frozen storage. J Food Sci 56:627–631
Leygonie C, Britz TJ, Hoffman LC (2012) Meat quality comparison between fresh and frozen/thawed ostrich M. iliofibularis. Meat Sci 91:364–368
Muela E, Sañudo C, Campo MM, Medel I, Beltrán JA (2010) Effect of freezing method and frozen storage duration on instrumental quality of lamb throughout display. Meat Sci 84:662–669
Privalov PL, Griko YV, Venyaminov SY, Kutyshenko VP (1986) Cold denaturation of myoglobin. J Mol Biol 190:487–498
Livingston DJ, Brown WD (1981) The chemistry of myoglobin and its reactions. Food Technol 35:238–252
Abdallah MB, Marchello JA, Ahmad HA (1999) Effect of freezing and microbial growth on myoglobin derivatives of beef. J Agric Food Chem 47:4093–4099
Kim YHB, Frandsen M, Rosenvold K (2011) Effect of ageing prior to freezing on colour stability of ovine Longissimus muscle. Meat Sci 88:332–337
Mancini RA, Hunt MC (2005) Current research in meat colour. Meat Sci 71:100–121
Wagner JR, Añón MC (1985) Effect of freezing rate on the denaturation of myofibrillar proteins. Int J Food Sci Technol 20:735–744
Estévez M (2011) Protein carbonyls in meat systems: a review. Meat Sci 89:259–279
Pettersen MK, Mielnik MB, Eie T, Skrede G, Nilsson A (2004) Lipid oxidation in frozen, mechanically deboned turkey meat as affected by packaging parameters and storage conditions. Poultry Sci 7:1240–1248
Xiong YL (2000) Protein oxidation and implications for muscle food quality. In: Decker E, Faustman C (eds) Antioxidants in muscle foods. Wiley, Chichester
Feeney RE, Blankenhorn G, Dixon BF (1975) Carbonyl-amine reactions in protein chemistry. Adv Protein Chem 29:135–203
Igene JO, Pearson AM, Dugan LR, Price JF (1980) Role of triglycerides and phospholipids on development of rancidity in model meat systems during frozen storage. Food Chem 5:263–276
Awad A, Powrie WD, Fennema O (1968) Chemical deterioration of frozen bovine muscle −4 °C. J Food Sci 33:227–234
James C, James SJ (2010) In: Todrá F (ed) Handbook of meat processing. Wiley-Blackwell, Ames
Department of Health (1994) Nutritional aspects of the cardiovascular disease. Report of health and social subjects 46, Her Majesty’s Stationery Office, London
De Smet S, Raes K, Demeyer D (2004) Meat fatty acid composition as affected by fatness and genetic factors: a review. Anim Res 53:81–98
Kanner J (1994) Oxidative processes in meat and meat products: quality implications. Meat Sci 36:169–189
Morrissey PA, Sheehy PJA, Galvin K, Kerry JP, Buckley DJ (1998) Lipid stability in meat and meat products. Meat Sci 49:S73–S86
Chaijan M (2008) Review: lipid and myoglobin oxidations in muscle foods. Songklanakarin J Sci Technol 30:47–53
Ledward DA (1970) Metmyoglobin formation in beef stored in carbon dioxide enriched and oxygen depleted atmospheres. J Food Sci 35:33–37
Huff-Lonergan E, Lonergan SM (2005) Mechanisms of water-holding capacity of meat: the role of postmortem biochemical and structural changes. Meat Sci 71:194–204
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The authors thank UVE S.A. for samples. We also thank the Animal Production personnel at the Faculty of Veterinary Science and especially Prof. M.M. Campo for their scientific assistance.
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Alonso, V., Muela, E., Tenas, J. et al. Changes in physicochemical properties and fatty acid composition of pork following long-term frozen storage. Eur Food Res Technol 242, 2119–2127 (2016). https://doi.org/10.1007/s00217-016-2708-y
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DOI: https://doi.org/10.1007/s00217-016-2708-y