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Identification of buffalo casein-derived bioactive peptides with osteoblast proliferation activity

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Abstract

Osteoblast cells are the building blocks of bone architecture and most suitable cells for screening osteoanabolic agents. As bone formation begins with osteoblast proliferation, it is the essential stage for screening osteoanbolics. LC–MS/MS analysis of the 11th RP–HPLC fraction of pepsin–trypsin (PT) hydrolysates of buffalo casein showed 15 peptides. Out of these 15 peptides, four peptides of less than 1 kDa were custom-synthesized and evaluated for osteoblast proliferating ability. Proliferation effect of the peptides was studied by formazan crystal formation (MTT method) and relative expression of proliferation marker genes (clay and cdk2). PT hydrolysates and all the four peptides significantly induced osteoblast proliferation indicated by both MTT and relative gene expression studies. This study showed the osteoblast-promoting property of peptides derived from milk proteins, thereby further substantiating the earlier epidemiological data regarding bone health-promoting effect of milk.

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Acknowledgments

The authors are grateful to the Director, ICAR-National Dairy Research Institute, Karnal, for providing funding and laboratory facilities to carry out this piece of work.

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Authors and Affiliations

  1. Animal Biochemistry Division, National Dairy Research Institute, Karnal, India

    Srinu Reddi, Suman Kapila & Rajeev Kapila

  2. Rajiv Gandhi Institute of Veterinary Education and Research, Kurumbapet, India

    Venkatesa Perumal Shanmugam

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  1. Srinu Reddi
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  2. Venkatesa Perumal Shanmugam
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  3. Suman Kapila
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  4. Rajeev Kapila
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Correspondence to Suman Kapila.

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Reddi, S., Shanmugam, V.P., Kapila, S. et al. Identification of buffalo casein-derived bioactive peptides with osteoblast proliferation activity. Eur Food Res Technol 242, 2139–2146 (2016). https://doi.org/10.1007/s00217-016-2710-4

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  • DOI: https://doi.org/10.1007/s00217-016-2710-4

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