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Analysis of CYP2C9*5 in Caucasian, Oriental and Black-African populations

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Abstract

Objective. CYP2C9 is a polymorphic gene with at least six known allelic variants (CYP2C9*1 to *6). CYP2C9*5 has been recently described in African-Americans. The lower activity of CYP2C9*5 encoded enzyme than *1 has been reported for the S-warfarin 7-hydroxylation in vitro. The aim of the present study was to develop an assay for the analysis of this variant and to determine the frequency of this polymorphism in different ethnic populations.

Materials and methods. A PCR-based endonuclease digestion method, using a mismatched forward primer that introduced a recognition site for AvaII in all the CYP2C9 genotypes except CYP2C9*5, is described. DNA samples from 150 Ethiopians, 183 Tanzanians, 200 Caucasians from Sweden and 150 Orientals from Korea were screened for this variant allele.

Results and conclusion. The CYP2C9*5 allele was analysed using a polymerase chain reaction-based endonuclease method, and it was found in three Tanzanians (allele frequency, 0.0082) but not in Ethiopians, Caucasians or Orientals.

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Authors and Affiliations

  1. Department of Medical Laboratory Sciences and Technology, Division of Clinical Pharmacology, C1–68, Karolinska Institutet at Huddinge University Hospital, SE-14186 Stockholm, Sweden, , , , ,

    Ümit Yasar, Roberto Canaparo, Mia Sandberg, Hyung-Keun Roh & Agneta Wennerholm

  2. Division of Molecular Toxicology, IMM, Karolinska Institutet, Stockholm, Sweden, , , , ,

    Eleni Aklillu

  3. Department of Clinical Pharmacology, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania, , , , ,

    Jane Sayi

Authors
  1. Ümit Yasar
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  2. Eleni Aklillu
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  3. Roberto Canaparo
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  4. Mia Sandberg
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  5. Jane Sayi
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  6. Hyung-Keun Roh
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  7. Agneta Wennerholm
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Yasar, Ü., Aklillu, E., Canaparo, R. et al. Analysis of CYP2C9*5 in Caucasian, Oriental and Black-African populations. Eur J Clin Pharmacol 58, 555–558 (2002). https://doi.org/10.1007/s00228-002-0518-6

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  • DOI: https://doi.org/10.1007/s00228-002-0518-6

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