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The Kinetics of Non-Lamellar Phase Formation in DOPE-Me: Relevance to Biomembrane Fusion

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Abstract

The mechanism of the lamellar/inverted cubic (Q II) phase transition is related to that of membrane fusion in lipid systems. N-Monomethylated dioleoylphosphatidylethanolamine (DOPE-Me) exhibits this transition and is commonly used to investigate the effects of exogenous substances, such as viral fusion peptides, on the mechanism of membrane fusion. We studied DOPE-Me phase behavior as a first step in evaluating the effects of membrane-spanning peptides on inverted phase formation and membrane fusion. These measurements show that: a) the onset temperatures for Q II and inverted hexagonal (H II) phase formation both are temperature scan rate-dependent; b) longer pre-incubation times at low temperature and lower temperature scan rates favor formation of the Q II phase; and c) in temperature-jump experiments between 61 and 65°C, the meta-stable H II phase forms initially, and disappears slowly while the Q II phase develops. These observations are rationalized in the context of a mechanism for both the lamellar/non-lamellar phase transition and the related process of membrane fusion.

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Acknowledgements

We are very grateful for the expert assistance of Dr. Malcolm S. Capel (National Synchrotron Light Source, Beamline X12B) in collecting the synchrotron TRXRD data. This project was supported by grants from the NIH (GM56969, GM61070) and the NSF (DIR 9016689, DBI 9981990).

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Authors and Affiliations

  1. Biochemistry, Biophysics and Chemistry, The Ohio State University, 100W 18th Ave., Columbus, OH 43210, USA

    V. Cherezov, D. P. Siegel & M. Caffrey

  2. Avanti Polar Lipids Inc., 700 Industrial Park Dr. Alabaster, AL 35007, USA

    W. Shaw & S. W. Burgess

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  1. V. Cherezov
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  2. D. P. Siegel
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  4. S. W. Burgess
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  5. M. Caffrey
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Correspondence to M. Caffrey.

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Cherezov, V., Siegel, D., Shaw, W. et al. The Kinetics of Non-Lamellar Phase Formation in DOPE-Me: Relevance to Biomembrane Fusion . J. Membrane Biol. 195, 165–182 (2003). https://doi.org/10.1007/s00232-003-0617-z

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  • DOI: https://doi.org/10.1007/s00232-003-0617-z

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