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Genetic Relatedness Among tdh + and trh + Vibrio parahaemolyticus Cultured from Gulf of Mexico Oysters (Crassostrea virginica) and Surrounding Water and Sediment

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Abstract

Pathogenic Vibrio parahaemolyticus (Vp) (tdh +/trh +) represent a small percentage of environmental Vp populations, and very little is known about this subpopulation. Repetitive extragenic palindromic PCR and multilocus sequence analysis revealed heterogeneity among 41 Vp containing thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) that were isolated from Mississippi coastal environments from October 2006 to April 2007. There was no source-specific sequestering in oysters, water, or sediment.

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Acknowledgements

Special thanks go to Jessica Jones and Read Hendon for manuscript review and graphics support, respectively. This study was funded by a NOAA Oceans and Human Health Initiative grant (NA-04-OAR4600214). Narjol Gonzalez-Escalona was supported by a grant from the U.S. Department of Agriculture, Cooperative State Research, Education and Extension Service, National Research Initiative, Competitive Grants Program, Epidemiological Approaches to Food Safety, Project #2004-35212-14882.

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Authors and Affiliations

  1. Gulf Coast Research Laboratory, University of Southern Mississippi, 703 East Beach Drive, Ocean Springs, MS, 39564, USA

    C. N. Johnson, A. R. Flowers, V. C. Young, N. F. Noriea III & D. J. Grimes

  2. Gulf Coast Seafood Laboratory, Food and Drug Administration, Dauphin Island, AL, USA

    A. DePaola

  3. Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USA

    N. Gonzalez-Escalona

Authors
  1. C. N. Johnson
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  2. A. R. Flowers
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  3. V. C. Young
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  4. N. Gonzalez-Escalona
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  5. A. DePaola
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  6. N. F. Noriea III
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  7. D. J. Grimes
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Correspondence to D. J. Grimes.

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Johnson, C.N., Flowers, A.R., Young, V.C. et al. Genetic Relatedness Among tdh + and trh + Vibrio parahaemolyticus Cultured from Gulf of Mexico Oysters (Crassostrea virginica) and Surrounding Water and Sediment. Microb Ecol 57, 437–443 (2009). https://doi.org/10.1007/s00248-008-9418-3

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  • DOI: https://doi.org/10.1007/s00248-008-9418-3

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