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Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain

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Abstract

We successfully demonstrated batch ethanol fermentation repeated ten times from raw starch with high ethanol productivity. We constructed a yeast diploid strain coexpressing the maltose transporter AGT1, α-amylase, and glucoamylase. The introduction of AGT1 allows maltose and maltotriose fermentation as well as the improvement of amylase activities. We also found that α-amylase activity during fermentation was retained by the addition of 10 mM calcium ion and that the highest α-amylase activity was 9.26 U/ml during repeated fermentation. The highest ethanol productivity was 2.22 g/l/h at the fourth batch, and after ten cycles, ethanol productivity of more than 1.43 g/l/h was retained, as was α-amylase activity at 6.43 U/ml.

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Acknowledgements

This work was supported by the Japanese Ministry of the Environment for Technical Development of Measures to Prevent Global Warming. This work was also partly supported by the Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan.

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Authors and Affiliations

  1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan

    Syun-ichi Yamakawa, Ryosuke Yamada, Chiaki Ogino & Akihiko Kondo

  2. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan

    Tsutomu Tanaka

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  1. Syun-ichi Yamakawa
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  2. Ryosuke Yamada
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  3. Tsutomu Tanaka
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  4. Chiaki Ogino
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  5. Akihiko Kondo
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Correspondence to Akihiko Kondo.

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Yamakawa, Si., Yamada, R., Tanaka, T. et al. Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain. Appl Microbiol Biotechnol 87, 109–115 (2010). https://doi.org/10.1007/s00253-010-2487-5

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  • DOI: https://doi.org/10.1007/s00253-010-2487-5

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