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Potentially Pathogenic Acanthamoeba Isolated from a Hospital in Brazil

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Abstract

Studies on free-living amoebae (FLA), has been increased in recent years, especially related to the genus Acanthamoeba, because these organisms are widely found in the environment. The present work isolated and characterized this organism from biofilms and dust in hospital environment. 135 samples were collected in 15 different environments in a hospital at the south of Brazil. Thirty-one (23%) isolates were identified as morphologically belonging to the Acanthamoeba genus and 10 of these were submitted to temperature and osmotolerance tests as criterion for evaluation of the viability and pathogenicity. The tests indicate that four (40%) of these isolates could be potentially pathogenic because grew at high temperature (40°C) and osmolarity (mannitol 1 M). Some isolates genotypes were determined after ribosomal DNA sequencing. These data revealed that three dust isolates belong to T4, two biofilm isolates to T5 and one dust isolate to T3 genotype. Therefore, Acanthamoeba found in the hospital environment represents a risk for people that circulate there.

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References

  1. Barbeau J, Buhler T (2001) Biofilms augment the number of free-living amoebae in dental unit waterlines. Res Microbiol 152:753–760

    Article  PubMed  CAS  Google Scholar 

  2. Barete S, Combes A, de Jonckheere JF, Datry A, Varnous S, Martinez V, Ptacek SG, Caumes E, Capron F, Frances C, Gibert C, Chasidow O (2007) Fatal disseminated Acanthamoeba lenticulata infection in a heart transplant patient. Emerg Infect Dis 13:736–738

    PubMed  CAS  Google Scholar 

  3. Booton GC, Rogerson A, Bonilla TD, Seal DV, Kelly DJ, Beattie TK, Tomlinson A, Lares-Villa F, Fuerst PA, Byers TJ (2004) Molecular and physiological evaluation of subtropical environmental isolates of Acanthamoeba spp. causal agent of Acanthamoeba keratitis. J Eukar Microbiol 51:192–200

    Article  CAS  Google Scholar 

  4. De Carli GA (2001) Parasitologia Clínica Seleção de Métodos e Técnicas de Laboratório para Diagnóstico das Parasitoses Humanas. São Paulo, Atheneu, pp 418–426

    Google Scholar 

  5. Declerck P, Behets J, van Hoef V, Ollevier F (2007) Detection of Legionella spp. and some of their amoeba hosts in floating biofilms from anthropogenic and natural aquatic environments. Water Res 41(14):3159–3167

    Article  PubMed  CAS  Google Scholar 

  6. Cabral FM, Cabral G (2003) Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 16:273–307

    Article  PubMed  Google Scholar 

  7. Garcia LS, Bruckner D (1993) Diagnostic medical parasitology, 2nd edn. American Society for Microbiology, Washington

    Google Scholar 

  8. Gautom RK, Lory S, Seyedirashti S, Bergeron DL, Fritsche TR (1994) Mitochondrial DNA fingerprinter of Acanthamoeba spp. isolated from clinical and environmental sources. J Clin Microbiol 32:1070–1073

    PubMed  CAS  Google Scholar 

  9. Khan NA (2006) Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev 30:564–595

    Article  PubMed  CAS  Google Scholar 

  10. Khan NA, Tareen NK (2003) Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains. F Parasitol 50:97–104

    CAS  Google Scholar 

  11. Khan NA, Jarroll EL, Paget TA (2001) Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. Curr Microbiol 43:204–208

    Article  PubMed  CAS  Google Scholar 

  12. Kong HH, Shin JY, Yu HS, Kim J, Hahn TW, Hahn YH, Chung DI (2002) Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents is southwestern Korea. J Clin Microbiol 40:1199–1206

    Article  PubMed  CAS  Google Scholar 

  13. Lorenzo-Morales J, Ortega-Rivas A, Foronda P, Martínez E, Valladares B (2005) Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources. Parasitol Res 95:273–277

    Article  PubMed  Google Scholar 

  14. Maghsood AH, Sissons J, Rezaian M, Nolder D, Warhurst D, Khan NA (2005) Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. J Med Microbiol 54:755–759

    Article  PubMed  CAS  Google Scholar 

  15. Mathers WD, Nelson SE, Lane JL, Wilson ME, Allen RC, Folberg R (2000) Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol 118:178–183

    PubMed  CAS  Google Scholar 

  16. Page FC (1988) A new key to freshwater and soil gymnamoebae with instructions for culture. Freshwater Biological Association, Ambleside, Cumbria

    Google Scholar 

  17. Penland RL, Wilhelmus KR (1997) Comparison of axenic and monoxenic media for isolation of Acanthamoeba. J Clin Microbiol 35:915–922

    PubMed  CAS  Google Scholar 

  18. Pussard R, Pons M (1977) Morphologie de le paroi kystique et taxonomíe du genere Acanthamoeba (Protozoa, Amoebida). Protistologica 4:557–598

    Google Scholar 

  19. Rivera F, Ramírez E, Bonilla P, Calderón A, Gallegos E, Rodríguez S, Ortiz R, Zaldívar B, Ramírez P, Durán (1993) A pathogenic and free-living amoebae isolated from swimming pools and physiotherapy tubs in Mexico. Environ Res 62:43–52

    Article  PubMed  CAS  Google Scholar 

  20. Rohr U, Weber S, Michel R, Selenka F, Wilhelm M (1998) Comparison of free-living amoebae in hot water systems of hospitals with isolates from moist sanitary areas by identifying genera and determining temperature tolerance. Appl Environ Microbiol 64:1822–1824

    PubMed  CAS  Google Scholar 

  21. Sambrook J, Fristsch EF, Maniats T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  22. Schroeder JM, Booton GC, Hay J, Niszl I, Seal DV, Markus MB, Fuerst PA, Byers TJ (2001) Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoeba from humans with keratitis and from sewage sludge. J Clin Microbiol 39:1903–1911

    Article  PubMed  CAS  Google Scholar 

  23. Schuster FL (2002) Cultivation of pathogenic and opportunistic free-living amebas. Clin Microbiol Rev 15:342–354

    Article  PubMed  Google Scholar 

  24. Schuster FL, Visvesvara GS (2004) Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. J Parasitol 34:1001–1027

    Google Scholar 

  25. Shukla OP, Kaul SM, Mehlotra RK (1990) Nutritional studies on Acanthamoeba culbertsoni and development of chemically defined medium. J Parasitol 37:237–242

    CAS  Google Scholar 

  26. Silva MA, Rosa JA (2003) Isolamento de amebas de vida livre potencialmente patogênica em poeira de hospitais. Revista de Saúde Pública 37:242–246

    PubMed  Google Scholar 

  27. Spanakos G, Tzanetou K, Miltsakakis D, Patsoula E, Malamou-Lada E, Vakalis NC (2006) Genotyping of pathogenic Acanthamoebae isolated from clinical samples in Greece—report of a clinical isolate presenting T5 genotype. Parasitol Int 55:147–149

    Article  PubMed  CAS  Google Scholar 

  28. Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodriguez-Zaragoza S, Dean CL, Fuerst PA, Byers TJ (1998) The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Eukar Microbiol 45:45–54

    Article  CAS  Google Scholar 

  29. Thomas V, Loret, Jousset M, Greub G (2008) Biodiversity of amoebae and amoebae- resisting bacteria in a drinking water treatment plant. Environ Microbiol 10:2728–2745

    Article  PubMed  CAS  Google Scholar 

  30. Tsvetkova N, Schild M, Panaiotov S, Kurdova-Mintcheva R, Gottstein B, Walonik J, Aspöck H, Lucas MS, Müller N (2004) The identification of free-living environmental isolates of amoebae from Bulgaria. Parasitol Res 92:405–413

    Article  PubMed  Google Scholar 

  31. Zhang Y, Sun X, Wang Z, Li R, Luo S, Jin X, Deng S, Chen W (2004) Identification of 18S ribosomal DNA genotype of Acanthamoeba from patients with keratitis in North China. Invest Ophthalmol Vis Sci 45:1904–1907

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, CEP 90050170, Brazil

    Ana Maris Carlesso & Geórgia Lazzari Artuso

  2. PPG Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, CEP 90050170, Brazil

    Karin Caumo

  3. Department of Microbiology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, CEP 90050170, Brazil

    Marilise Brittes Rott

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  1. Ana Maris Carlesso
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  2. Geórgia Lazzari Artuso
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  3. Karin Caumo
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  4. Marilise Brittes Rott
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Correspondence to Marilise Brittes Rott.

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Carlesso, A.M., Artuso, G.L., Caumo, K. et al. Potentially Pathogenic Acanthamoeba Isolated from a Hospital in Brazil. Curr Microbiol 60, 185–190 (2010). https://doi.org/10.1007/s00284-009-9523-7

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  • DOI: https://doi.org/10.1007/s00284-009-9523-7

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