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Enterobacter Strains Might Promote Colon Cancer

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Abstract

Many studies have been performed to determine the interaction between bacterial species and cancer. However, there has been no attempts to demonstrate a possible relationship between Enterobacter spp. and colon cancer so far. Therefore, in the present study, it is aimed to investigate the effects of Enterobacter strains on colon cancer. Bacterial proteins were isolated from 11 Enterobacter spp., one Morganella morganii, and one Escherichia coli strains, and applied onto NCM460 (Incell) and CRL1790 (ATCC) cell lines. Cell viability and proliferation were determined in MTS assay. Flow Cytometry was used to detect CD24 level and apoptosis. Real-Time PCR studies were performed to determine NFKB and Bcl2 expression. Graphpad Software was used for statistical analysis. The results showed that proteins, isolated from the Enterobacter spp., have significantly increased cell viability and proliferation, while decreasing the apoptosis of the cell lines tested. The data in the present study indicated that Enterobacter strains might promote colon cancer. Moreover, Enterobacter spp. could be a clinically important factor for colon cancer initiation and progression. Studies can be extended on animal models in order to develop new strategies for treatment.

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Acknowledgements

The authors are thankful to Ali Umman Doğan, Nilsu Atay, Assoc. Prof. Dr. Dilek Telci, Prof. Dr. Ufuk Hasdemir, Prof. Dr. Necmettin Sökücü, Prof. Dr. Adile Çevikbaş, and Assoc. Prof. Dr. Ümran Soyoğul Gürer for their help and support. The authors are grateful to INCELL Corporation, LLC for providing NCM460 Cell Lines. The authors also would like to thank Yeditepe University, Genetics and Bioengineering Department and Istanbul Technical University, Scientific Research Foundations.

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Authors and Affiliations

  1. Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey

    Dilşad Yurdakul & Fikrettin Şahin

  2. Molecular Biology and Genetics Department and Molecular Biology, Biotechnology and Genetics Research Center (MOBGAM), Istanbul Technical University, Istanbul, Turkey

    Ayten Yazgan-Karataş

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  1. Dilşad Yurdakul
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Correspondence to Fikrettin Şahin.

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Yurdakul, D., Yazgan-Karataş, A. & Şahin, F. Enterobacter Strains Might Promote Colon Cancer. Curr Microbiol 71, 403–411 (2015). https://doi.org/10.1007/s00284-015-0867-x

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