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Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall

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Abstract

The exoproteome of the fungus Fusarium graminearum grown on glucose and on hop (Humulus lupulus, L.) cell wall has been investigated. The culture medium was found to contain a higher quantity of proteins and the proteins are more diverse when the fungus is grown on cell wall. Using both 1D and 2D electrophoresis followed by mass spectrometry analysis and protein identification based on similarity searches, 84 unique proteins were identified in the cell wall-grown fungal exoproteome. Many are putatively implicated in carbohydrate metabolism, mainly in cell wall polysaccharide degradation. The predicted carbohydrate-active enzymes fell into 24 different enzymes classes, and up to eight different proteins within a same class are secreted. This indicates that fungal metabolism becomes oriented towards synthesis and secretion of a whole arsenal of enzymes able to digest almost the complete plant cell wall. Cellobiohydrolase is one of the only four proteins found both after growth on glucose and on plant cell wall and we propose that this enzyme could act as a sensor of the extracellular environment. Extensive knowledge of this very diverse F. graminearum exoproteome is an important step towards the full understanding of Fusarium/plants interactions.

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Acknowledgements

This work was supported by the Cophoudal (Brumath, France) and by the French agency for agriculture development (ADAR). Didier Hatsch was funded by a Ph.D. fellowship from the Region Alsace. We thank the Bruker Daltonics society and the CNRS for Christine Carapito’s Ph.D. fellowship and Aventis for François Delalande’s post-doc fellowship. Anne Forster and Daniéle Thierse are greatly acknowledged for their technical competence. We are grateful to Prof. Pierre Oudet and Jan DeMey for helpful and interesting discussions and materials. The work performed at Cambridge was supported by grants from the BBSRC.

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Authors and Affiliations

  1. UMR 7175—Laboratoire de Phytopathologie, Université Louis Pasteur, Illkirch, France

    Vincent Phalip, Didier Hatsch & Jean-Marc Jeltsch

  2. UMR 7512—Science Analytique et Interactions Ioniques, Moléculaires et Biomoléculaires, Université Louis Pasteur, Strasbourg, France

    François Delalande, Christine Carapito, Emmanuelle Leize-Wagner & Alain Van Dorsselaer

  3. Department of Biochemistry, University of Cambridge, Cambridge, UK

    Florence Goubet & Paul Dupree

  4. Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brandt, BP 10413, 67412, Illkirch-Graffenstaden Cedex, France

    Vincent Phalip

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  1. Vincent Phalip
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  2. François Delalande
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Correspondence to Vincent Phalip.

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Phalip, V., Delalande, F., Carapito, C. et al. Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall. Curr Genet 48, 366–379 (2005). https://doi.org/10.1007/s00294-005-0040-3

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  • DOI: https://doi.org/10.1007/s00294-005-0040-3

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