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A proteomic view into infection of greyback canegrubs (Dermolepida albohirtum) by Metarhizium anisopliae

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Abstract

Metarhizium anisopliae is a naturally occurring cosmopolitan fungus infecting greyback canegrubs (Dermolepida albohirtum). The main molecular factors involved in the complex interactions occurring between the greyback canegrubs and M. anisopliae (FI-1045) were investigated by comparing the proteomes of healthy canegrubs, canegrubs infected with Metarhizium and fungus only. Differentially expressed proteins from the infected canegrubs were subjected to mass spectrometry to search for pathogenicity related proteins. Immune-related proteins of canegrubs identified in this study include cytoskeletal proteins (actin), cell communication proteins, proteases and peptidases. Fungal proteins identified include metalloproteins, acyl-CoA, cyclin proteins and chorismate mutase. Comparative proteome analysis provided a view into the cellular reactions triggered in the canegrub in response to the fungal infection at the onset of biological control.

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Authors and Affiliations

  1. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, 2109, Australia

    Nirupama Shoby Manalil, Valentino S. Junior Te’o & K. M. Helena Nevalainen

  2. Bureau of Sugar Experiment Stations Limited, Meiers Road, Indooroopilly, QLD, 4068, Australia

    Kathy Braithwaite & Stevens Brumbley

  3. Bureau of Sugar Experiment Stations Limited, Mackay, QLD, Australia

    Peter Samson

Authors
  1. Nirupama Shoby Manalil
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  2. Valentino S. Junior Te’o
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  3. Kathy Braithwaite
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  4. Stevens Brumbley
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  5. Peter Samson
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  6. K. M. Helena Nevalainen
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Correspondence to Nirupama Shoby Manalil.

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Communicated by J. Heitman.

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Manalil, N.S., Junior Te’o, V.S., Braithwaite, K. et al. A proteomic view into infection of greyback canegrubs (Dermolepida albohirtum) by Metarhizium anisopliae . Curr Genet 55, 571–581 (2009). https://doi.org/10.1007/s00294-009-0270-x

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  • DOI: https://doi.org/10.1007/s00294-009-0270-x

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