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BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea

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Abstract

Monocarboxylate transporters have a central role in mammalian metabolism, but rarely reported in phytopathogenic fungi. In this study, a putative monocarboxylate transporter gene in Botrytis cinerea [B. cinerea MctA (BcMctA)] was identified in the research of a B. cinerea transfer DNA (T-DNA) insertional mutant (74). Disruption of the gene decreased the growth rate on the medium with monocarboxylate (acetate or pyruvate) as the sole carbon sources, but not affected on lactate. The pyruvate contents in BcmctA deletion mutants decreased about 35 % compared with the wild strain. Besides, the conidial yield was increased about two times in BcmctA disruption mutant. The pathogenicity assay indicated that disruption of BcmctA significantly reduced the virulence of B. cinerea on cucumber and tomato leaves. Our results demonstrated that BcMctA is related to pyruvate uptake and pathogenicity of B. cinerea on cucumber and tomato leaves.

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Acknowledgments

This work was supported by a Grant from the Scientific Research Foundation of Zhejiang Province (No. LY12C14008) and National Nature Science Foundation of China (No. 31272002).

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Authors and Affiliations

  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Zhifeng Cui, Nana Gao, Qian Wang, Yun Ren, Kun Wang & Tingheng Zhu

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  1. Zhifeng Cui
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  2. Nana Gao
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  3. Qian Wang
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  4. Yun Ren
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  5. Kun Wang
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  6. Tingheng Zhu
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Correspondence to Zhifeng Cui or Tingheng Zhu.

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Communicated by M. Kupiec.

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Cui, Z., Gao, N., Wang, Q. et al. BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea . Curr Genet 61, 545–553 (2015). https://doi.org/10.1007/s00294-015-0474-1

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  • DOI: https://doi.org/10.1007/s00294-015-0474-1

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