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Responses of rose RhACS1 and RhACS2 promoters to abiotic stresses in transgenic Arabidopsis thaliana

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Abstract

Key message

Promoter activities of RhACS1 and RhACS2 , two rose genes involved in ethylene biosynthesis, are highly sensitive to various abiotic stresses in an organ-specific manner.

Abstract

Our previous studies indicated that two rose (Rosa hybrida) 1-aminocyclopropane-1-carboxylic acid synthase genes, RhACS1 and RhACS2, play a role in dehydration-induced ethylene production and inhibition of cell expansion in rose petals. Here, both RhACS1 and RhACS2 promoters were analyzed using histochemical staining and glucuronidase synthase (GUS) gene reporter activity assays following their introduction into transgenic Arabidopsis thaliana plants. It was found that the promoter activities of both genes were strong throughout the course of development from young seedlings to mature flowering plants in various organs, including hypocotyls, cotyledons, leaves, roots and lateral roots. RhACS1 promoter activity was induced by drought in both rosette leaves and roots of transgenic A. thaliana lines, but was reduced following a re-hydration treatment. In contrast, RhACS2 promoter activity was decreased by drought in rosette leaves, while its response pattern was similar to that of RhACS1 in roots. A mannitol treatment induced the activity of both the RhACS1 and RhACS2 promoters, indicating that both genes are also regulated by osmotic stress. In addition, RhACS2 appeared to be abscisic acid (ABA)-inducible, while RhACS1 was less sensitive to ABA. Finally, four truncated sequences of the RhACS1 promoter were generated and GUS activity assays demonstrated that deleting a 327 bp region between bp 862 and −535 resulted in a substantial decrease of the promoter activity. Taken together, our results suggest that the RhACS1 and RhACS2 promoters respond to abiotic stresses in a developmentally regulated and spatially specific manner.

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Abbreviations

ABA:

Abscisic acid

ACC:

1-aminocyclopropane-1-carboxylic acid

ACS:

ACC synthase

CHX:

Cycloheximide

GUS:

β-glucuronidase

IAA:

Indole acetic acid

MS:

Murashige and Skoog

SAM:

S-adenosylmethionine

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Acknowledgments

This work was supported by the National Natural Science Foundation of China, Grant 31372095 and Grant 31130048, and the 948 project (2011-G17) of the Ministry of Agriculture. We thank PlantScribe (www.plantscribe.com) for carefully editing this manuscript.

Conflict of interest

All the authors declared that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Ornamental Horticulture, China Agricultural University, Beijing, 100193, China

    Muhammad Ali Khan, Yonglu Meng, Daofeng Liu, Hongshu Tang, Suhui Lü, Muhammad Imtiaz, Guimei Jiang, Peitao Lü, Yuqi Ji, Junping Gao & Nan Ma

  2. The University of Agriculture Peshawar, Peshawar, 25000, Pakistan

    Muhammad Ali Khan & Muhammad Imtiaz

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  1. Muhammad Ali Khan
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  2. Yonglu Meng
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Correspondence to Nan Ma.

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Communicated by R. Schmidt.

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Khan, M.A., Meng, Y., Liu, D. et al. Responses of rose RhACS1 and RhACS2 promoters to abiotic stresses in transgenic Arabidopsis thaliana . Plant Cell Rep 34, 795–804 (2015). https://doi.org/10.1007/s00299-015-1742-8

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  • DOI: https://doi.org/10.1007/s00299-015-1742-8

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