Abstract
The japonica rice variety Taipei 309 was cotransformed by particle bombardment of immature embryo-derived embryogenic calli with a modified δ-endotoxin gene cryIA(b) of Bacillus thuringiensis (Bt) under the control of the rice Actin1 promoter, and the hygromycin resistance gene, hph driven by the CaMV35S promoter. Selected transgenic rice plants showed enhanced insecticidal activity against yellow stem borer (Scirpophaga incertulas), with mortality rates reaching up to 100% in a bioassay with cut stems. Introduction and expression of the Actin1 promoter-Bt gene into rice provides japonica rice germplasm resistant to insect attack.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 21 March 1997 / Revision received: 23 June 1997 / Accepted: 5 July 1997
Rights and permissions
About this article
Cite this article
Wu, C., Fan, Y., Zhang, C. et al. Transgenic fertile japonica rice plants expressing a modified cryIA(b) gene resistant to yellow stem borer. Plant Cell Reports 17, 129–132 (1997). https://doi.org/10.1007/s002990050365
Issue Date:
DOI: https://doi.org/10.1007/s002990050365