Abstract
Detection of a single cured structure in two-photon photopolymerization (TPP) based microstructure fabrication requires the laser focal spot to be exactly positioned on the cover slip. This is due to the fact that if the laser focal position is not exactly on the cover slip, the structure may not stick to the cover slip and flow away with the liquid during the washing and developing stages. In this paper, we report a scheme of laser spot positioning for the implementation of TPP process and the detection of a single cured microstructure under an optical microscope. For this, a novel yet very simple approach is devised and an uncomplicated procedure is developed. Experimental results are also included to prove the worthiness of the devised method.
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Acknowledgments
This work (2012R1A2A2A03045928) was supported by Mid-career Researcher Program through National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) and by the Basic Research Project through a grant provided by GIST.
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Najam, M.T.B., Arif, K.M. & Lee, YG. Novel method for laser focal point positioning on the cover slip for TPP-based microfabrication and detection of the cured structure under optical microscope. Appl. Phys. B 111, 141–147 (2013). https://doi.org/10.1007/s00340-012-5317-x
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DOI: https://doi.org/10.1007/s00340-012-5317-x