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Tobacco bacterial wilt can be biologically controlled by the application of antagonistic strains in combination with organic fertilizer

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Abstract

Bacterial wilt caused by Ralstonia solanacearum is one of the most serious tobacco diseases worldwide. Brevibacillus brevis (L-25) and Streptomyces rochei (L-9) with strong inhibitory effects on R. solanacearum in vitro were isolated from the rhizosphere of a healthy tobacco plant in a severely wilt-diseased field. Pot and field experiments were conducted to evaluate the biocontrol effect of the isolated antagonists alone and in combination with organic fertilizer. In pot experiment, the control efficacy was 92.3–100 % in the treatments applied with L-25 and L-9 alone or together with organic fertilizers. When bioorganic fertilizer containing L-9 and L-25 was applied to the soil in field condition, the control efficacies were 95.4 and 30.0 in the Anhui and Guizhou field plots, respectively. The counts of bacteria and actinomycetes in rhizosphere soil were significantly increased (p ≤ 0.05) under all antagonist applications compared with CK (PR). In contrast, fungal and R. solanacearum densities in the rhizosphere soil applied with antagonists were much lower than the CK (PR) rhizosphere. Combined application of the two antagonists had better effect than single antagonist treatments. The antagonists were more effective when they were combined with organic fertilizer as compared with the antagonistic strains only. These results allow us to conclude that a combination of the biocontrol agents, L-25 and L-9, together with organic fertilizers can effectively control bacterial wilt by affecting soil microbial structure.

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Acknowledgments

This study was financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), by 111 project (B12009), and by the Agricultural Ministry of China (201103004).

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  1. Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China

    Yanxia Liu, Xingming Yang, Xiang Li & Qirong Shen

  2. Guizhou Tobacco Institute, Guiyang, 550000, China

    Yanxia Liu, Junxiong Shi, Yonggang Feng & Xiang Li

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Liu, Y., Shi, J., Feng, Y. et al. Tobacco bacterial wilt can be biologically controlled by the application of antagonistic strains in combination with organic fertilizer. Biol Fertil Soils 49, 447–464 (2013). https://doi.org/10.1007/s00374-012-0740-z

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