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Hypoglycaemia causes degeneration of large myelinated nerve fibres in the vagus nerve of insulin-treated diabetic BB/Wor rats

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Abstract

The aim of this study was to find out whether dysglycaemia causes neuropathy in the vagus nerve of insulin-treated diabetic BB/Wor rats. Specimens were collected from the left vagus nerve proximal and distal to the level of recurrent laryngeal branch and from the recurrent branch itself in control rats and diabetic BB/Wor rats subjected to hyper- or hypoglycaemia. Myelinated and unmyelinated axons were counted and myelinated axon diameters were measured by electron microscopy. In controls, the vagus nerve proximal to the recurrent branch exhibited three regions in terms of fibre composition: part A was mainly composed of large myelinated axons, part B contained small myelinated and unmyelinated axons, and part C contained mainly unmyelinated axons. The distal level resembled part C at the proximal level and the recurrent branch resembled parts A and B. In hyperglycaemic rats, a normal picture was found at the proximal and distal levels of the vagus nerve and in the recurrent branch. In hypoglycaemic rats, signs of past and ongoing degeneration and regeneration of large myelinated axons were found at the proximal and distal levels and in the recurrent branch. We conclude that hypoglycaemia elicits degenerative alterations in large myelinated axons in the vagus and recurrent laryngeal nerves in diabetic BB/Wor rats. The absence of signs of neuropathy in unmyelinated and small myelinated axons suggests that the sensory and autonomic components of the nerve are less affected. In contrast, the hyperglycaemic rats examined here did not show obvious degenerative alterations.

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Acknowledgements

This study was supported by grants from the Swedish Science Council (Proj. No. 3761), the County Council of Östergötland, and by the Faculty of Health Sciences, Linköping University. Comments on the manuscript by Prof. Claes Hildebrand are gratefully acknowledged.

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  1. Division of Cell Biology, Department of Biomedicine and Surgery and Diabetic Research Centre, Faculty of Health Sciences, Linköping University, 581 85 , Linköping, Sweden

    Reza Jamali & Simin Mohseni

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  1. Reza Jamali
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Correspondence to Simin Mohseni.

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Jamali, R., Mohseni, S. Hypoglycaemia causes degeneration of large myelinated nerve fibres in the vagus nerve of insulin-treated diabetic BB/Wor rats. Acta Neuropathol 109, 198–206 (2005). https://doi.org/10.1007/s00401-004-0932-1

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  • DOI: https://doi.org/10.1007/s00401-004-0932-1

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