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Genetic association of IFN-γ +874T/A polymorphism in Mexican patients with drug-induced Stevens-Johnson syndrome/toxic epidermal necrolysis

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Abstract

Stevens–Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) are rare, but potentially life-threatening diseases, characterized by widespread epidermal necrosis and are predominantly drug induced. There is a paucity of data regarding the role of cytokine and cytokine receptors polymorphisms in the pathoimmunology of SJS/TEN. The aim of this study was to investigate the role of TNF-α-308, IFN-γ +874, IL-10-1082, IL-13 Arg130Gln, and IL-4R Gln551Arg gene polymorphisms in SJS/TEN in Mexican Mestizo patients. Twenty-nine unrelated SJS/TEN patients and 128 unrelated healthy individuals were studied. Genomic extraction was carried out from complete blood samples using the salting out method. The PCR–RFLP method was used to amplify the following polymorphisms: TNF-α-308, IFN-γ +874, IL-10-1082, IL-13 Arg130Gln, and IL-4R Gln551Arg. TNF-α-308, IL-10-1082, IL-13 Arg130Gln, and IL-4R Gln551Arg polymorphisms were not associated with the genetic susceptibility to SJS/TEN. The distribution of TT, TA, AA genotypes of IFN-γ +874 was significantly different in SJS/TEN patients compared with controls (pC = 0.012). TA and AA genotypes were grouped to highlight the differences between patients and controls given by the absence of the AA genotype in the group of patients (pC = 0.03, OR = 3.61 95 % CI 1.20–11.6). This preliminary study suggests that IFN-γ +874 T/A polymorphism is associated with SJS/TEN.

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Acknowledgments

The authors gratefully acknowledge Drs. Sagrario Hierro Orozco, Monserrat Solar Macías, Andrés Tirado Sánchez, Valeria Díaz Molina and Yadira Rodríguez Míguez MSc for their contribution in collecting blood samples for genomic screening.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Dermatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico

    Yann Charli-Joseph & Rocío Orozco-Topete

  2. Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15. Tlalpan, México DF, 14000, Mexico

    Guadalupe Lima, Dolores Ramos-Bello & Luis Llorente

  3. Department of Pathology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico

    Diana Aguilar

Authors
  1. Yann Charli-Joseph
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  2. Guadalupe Lima
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  3. Dolores Ramos-Bello
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  4. Diana Aguilar
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  5. Rocío Orozco-Topete
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  6. Luis Llorente
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Correspondence to Luis Llorente.

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Charli-Joseph, Y., Lima, G., Ramos-Bello, D. et al. Genetic association of IFN-γ +874T/A polymorphism in Mexican patients with drug-induced Stevens-Johnson syndrome/toxic epidermal necrolysis. Arch Dermatol Res 305, 353–357 (2013). https://doi.org/10.1007/s00403-012-1305-6

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  • DOI: https://doi.org/10.1007/s00403-012-1305-6

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