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Inhibition of UVA-induced apoptotic signaling pathway by polypeptide from Chlamys farreri in human HaCaT keratinocytes

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Abstract

Chronic UVA irradiation has been reported to induce photoaging and photocarcinogenesis. UVA is a potent inducer of reactive oxygen species (ROS), which can induce various biological processes, including apoptosis. Polypeptide from Chlamys farreri (PCF) is a novel marine active material isolated from the gonochoric Chinese scallop C. farreri. In our previous studies, PCF was found to be an effective antioxidant inhibiting UVA-induced ROS production and a potential inhibitory agent for UVA-induced apoptosis in the human keratinocyte cell line HaCaT. The intracellular mechanisms of how PCF protects HaCaT cells from UVA-induced apoptosis are not understood. Thus, we here investigate the effect of PCF on UVA-induced intracellular signaling of apoptosis. Pretreatment with the ROS scavenger N-acetylcysteine (NAC), the p38 MAPK inhibitor SB203580 or the caspase-3 inhibitor Ac-DEVD-CHO was found to effectively prevent UVA-induced apoptosis, indicating that ROS, p38 MAPK and caspase-3 play important roles in apoptosis. H2O2-induced apoptosis was attenuated by PCF, suggesting that PCF plays its anti-apoptotic role through its antioxidant activity. In addition, PCF treatment inhibited UVA-induced p38 MAPK activation and caspase-3 activation, as assayed by Western blot analysis and flow cytometry, respectively. Our results suggest that PCF attenuates UVA-induced apoptosis through a reduction of ROS generation and diminished p38 MAPK and caspase-3 activation.

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References

  1. Offord EA, Gautier JC, Avanti O, Scaletta C, Runge F, Kramer K, Applegate LA (2002) Photoprotective potential of lycopene, beta-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts. Free Radic Biol Med 32:1293–1303

    Article  Google Scholar 

  2. de Gruijl FR (2000) Photocarcinogenesis: UVA vs. UVB. Methods Enzymol 319:359–366

    Article  Google Scholar 

  3. Matsui MS, DeLeo VA (1991) Longwave ultraviolet radiation and promotion of skin cancer. Cancer Cells 3:8–12

    Google Scholar 

  4. de Laat A, van der Leun JC, de Gruijl FR (1997) Carcinogenesis induced by UVA (365-nm) radiation: the dose–time dependence of tumor formation in hairless mice. Carcinogenesis 18:1013–1020

    Article  Google Scholar 

  5. Sterenborg HJ, van der Leun JC (1990) Tumorigenesis by a long wavelength UV-A source. Photochem Photobiol 51:325–330

    Google Scholar 

  6. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T (2003) UV-induced skin damage. Toxicology 189:21–39

    Article  Google Scholar 

  7. Buttke TM, Sandstrom PA (1994) Oxidative stress as a mediator of apoptosis. Immunol Today 15:7–10

    Article  Google Scholar 

  8. Obata T, Brown GE, Yaffe MB (2000) MAP kinase pathways activated by stress: the p38 MAPK pathway. Crit Care Med 28:N67–N77

    Article  Google Scholar 

  9. Shimizu H, Banno Y, Sumi N, Naganawa T, Kitajima Y, Nozawa Y (1999) Activation of p38 mitogen-activated protein kinase and caspases in UVB-induced apoptosis of human keratinocyte HaCaT cells. J Invest Dermatol 112:769–774

    Article  Google Scholar 

  10. Assefa Z, Vantieghem A, Garmyn M, Declercq W, Vandenabeele P, Vandenheede JR, Bouillon R, Merlevede W, Agostinis P (2000) p38 mitogen-activated protein kinase regulates a novel, caspase-independent pathway for the mitochondrial cytochrome c release in ultraviolet B radiation-induced apoptosis. J Biol Chem 275:21416–21421

    Article  Google Scholar 

  11. Hildesheim J, Awwad RT, Fornace AJ Jr (2004) p38 Mitogen-activated protein kinase inhibitor protects the epidermis against the acute damaging effects of ultraviolet irradiation by blocking apoptosis and inflammatory responses. J Invest Dermatol 122:497–502

    Article  Google Scholar 

  12. Patel T, Gores GJ, Kaufmann SH (1996) The role of proteases during apoptosis. FASEB J 10:587–597

    Google Scholar 

  13. Kimura C, Zhao QL, Kondo T, Amatsu M, Fujiwara Y (1998) Mechanism of UV-induced apoptosis in human leukemia cells: roles of Ca2+/Mg2+-dependent endonuclease, caspase-3, and stress-activated protein kinases. Exp Cell Res 239:411–422

    Article  Google Scholar 

  14. Dou M, Han Y, Han Z, Chen X, Wang Y, Tan J, Wang C, Hou Y (2004) Inhibitory effect of polypeptide from Chlamys farreri on UVA induced apoptosis in human keratinocytes. Invest New Drugs 22:391–398

    Article  Google Scholar 

  15. Khodarev NN, Sokolova IA, Vaughan AT (1998) Mechanisms of induction of apoptotic DNA fragmentation. Int J Radiat Biol 73:455–467

    Article  Google Scholar 

  16. Chung JH, Han JH, Hwang EJ, Seo JY, Cho KH, Kim KH, Youn JI, Eun HC (2003) Dual mechanisms of green tea extract (EGCG)-induced cell survival in human epidermal keratinocytes. FASEB J 10:1913–1915

    Google Scholar 

  17. Tarozzi A, Marchesi A, Hrelia S, Angeloni C, Andrisano V, Fiori J, Cantelli-Forti G, Hrelia P (2005) Protective effects of cyanidin-3-O-beta-glucopyranoside against UVA-induced oxidative stress in human keratinocytes. Photochem Photobiol 81:623–629

    Article  Google Scholar 

  18. Yao RY, Wang CB (2002) Protective effects of Polypeptide from Chlamys farreri on Hela cells damaged by ultraviolet A. Acta Pharmacol Sin 23:1018–1022

    MathSciNet  Google Scholar 

  19. Han YT, Han ZW, Yu GY, Wang YJ, Cui RY, Wang CB (2004) Inhibitory effect of polypeptide from Chlamys farreri on ultraviolet A induced oxidative damage on human skin fibroblasts in vitro. Pharmacol Res 49:265–274

    Article  Google Scholar 

  20. Lu SC (2000) Regulation of glutathione synthesis. Curr Topics Cell Reg 36:95–116

    Article  Google Scholar 

  21. He YY, Hader DP (2002) UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.: protective effects of ascorbic acid and N-acetyl-l-cysteine. J Photochem Photobiol B 66:115–124

    Article  Google Scholar 

  22. Slyshenkov VS, Piwocka K, Sikora E, Wojtczak L (2001) Pantothenic acid protects jurkat cells against ultraviolet light-induced apoptosis. Free Radic Biol Med 30:1303–1310

    Article  Google Scholar 

  23. Peus D, Vasa RA, Beyerle A, Meves A, Krautmacher C, Pittelkow MR (1999) UVB activates ERK1/2 and p38 signaling pathways via reactive oxygen species in cultured keratinocytes. J Invest Dermatol 112:751–756

    Article  Google Scholar 

  24. Kabuyama Y, Homma MK, Sekimata M, Homma Y (2001) Wavelength-specific activation of MAP kinase family proteins by monochromatic UV irradiation. Photochem Photobiol 73:147–152

    Article  Google Scholar 

  25. Ding M, Li J, Leonard SS, Shi X, Costa M, Castranova V, Vallyathan V, Huang C (2002) Differential role of hydrogen peroxide in UV-induced signal transduction. Mol Cell Biochem 234–235:81–90

    Article  Google Scholar 

  26. Bachelor MA, Bowden GT (2004) Ultraviolet A-induced modulation of bcl-xl by p38 MAPK in human keratinocytes: post-transctiotional regulation through the 3′ untranslated region. J Biol Chem 279:42658–42668

    Article  Google Scholar 

  27. Tada-Oikawa S, Oikawa S, Kawanishi S (1998) Role of ultraviolet A-induced oxidative DNA damage in apoptosis via loss of mitochondrial membrane potential and caspase-3 activation. Biochem Biophys Res Commun 247:693–696

    Article  Google Scholar 

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Acknowledgments

The work was supported by the National Science Natural Foundation of China (No. 30471458) and Science Foundation of Shandong province (No. Y2003c02).

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Authors and Affiliations

  1. 422 Room, Boya Building, Medical College, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China

    Jin-Lian Li, Ning Liu, Xue-Hong Chen & Chun-Bo Wang

  2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Mi Sun

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  1. Jin-Lian Li
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  2. Ning Liu
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Correspondence to Chun-Bo Wang.

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Li, JL., Liu, N., Chen, XH. et al. Inhibition of UVA-induced apoptotic signaling pathway by polypeptide from Chlamys farreri in human HaCaT keratinocytes. Radiat Environ Biophys 46, 263–268 (2007). https://doi.org/10.1007/s00411-007-0112-5

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  • DOI: https://doi.org/10.1007/s00411-007-0112-5

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