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The effect of reactive oxidant generation in acute exercise-induced proteinuria in trained and untrained rats

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Abstract

Exercise-induced proteinuria is a common consequence of physical activity, although its mechanism is not clear. We investigated whether free radicals generated during exercise play a role in post-exercise proteinuria in sedentary and treadmill-running trained rats, separately. Sedentary and trained rats were randomly divided into four sub-groups: control, antioxidant treatment, exhaustive exercise and an exhaustive exercise plus antioxidant treatment group. Antioxidant therapy was applied by intragastric catheter for 4 weeks with vitamin C (ascorbic acid, 50 mg·kg−1·day−1) and vitamin E (α-tocopherol, 20 mg·kg−1·day−1). Twenty-four-hour urine samples were used for measuring protein levels and protein electrophoresis. Thiobarbituric acid (TBARS) and glutathione (GSH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were assayed in blood and tissues. Increased urinary protein levels and mixed type proteinuria in electrophoresis were identified after exhaustive exercise in sedentary rats. Erythrocyte, kidney and muscle TBARS levels were significantly elevated in this group. Antioxidant treatment prevented the increase in urinary protein levels, TBARS levels and the occurrence of mixed type proteinuria after exhaustive exercise in sedentary rats. Exhaustive exercise in trained rats resulted in elevation of urine protein levels and mixed type proteinuria although kidney TBARS levels were not changed compared to those of the trained controls. Antioxidant therapy in trained and exhausted-trained animals resulted in decreased TBARS levels in the kidney but it did not affect urinary-increased protein levels or electrophoresis in exhausted animals. This findings suggest that the exercise-induced oxidant stress may contribute to post-exercise proteinuria in sedentary rats. However, this mechanism may not be responsible for proteinuria in trained rats.

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Acknowledgements

This study was supported by a grant from Akdeniz University Research Foundation (99.01.0103.07) and complies with the current laws for research with experimental animals in Turkey. We thank Mrs. Sally Erkul from Agora Language Company for correcting the English of the manuscript.

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  1. Department of Physiology, Medical Faculty, Akdeniz University, Kampus, 07070, Antalya, Turkey

    Filiz Gündüz & Ümit Kemal Şentürk

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  1. Filiz Gündüz
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  2. Ümit Kemal Şentürk
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Correspondence to Ümit Kemal Şentürk.

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Gündüz, F., Şentürk, Ü.K. The effect of reactive oxidant generation in acute exercise-induced proteinuria in trained and untrained rats. Eur J Appl Physiol 90, 526–532 (2003). https://doi.org/10.1007/s00421-003-0888-1

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