Abstract
Purpose
To examine the effects of 24-h controlled carbohydrate intake on next day pre- and post-exercise inflammatory and hepcidin responses.
Methods
In a crossover design, 12 well-trained endurance athletes (Ht 181.08 ± 7.68 cm; Wt 74.8 ± 11.5 kg, VO2peak 68.9 ± 7.2 ml kg−1 min−1) completed two experimental (2-day) trials. On day 1, participants completed a glycogen depletion task, including a 16-km run (80 % vVO2peak) and 5 × 1 min efforts (130 % vVO2peak) separated by 2-min recovery. Subsequently, strict dietary control was enforced for 24 h, where low carbohydrate (LCHO 3 g kg−1) or high carbohydrate (HCHO 10 g kg−1) diets were provided. Twenty-four hours later, participants completed an 8 × 3 min interval running session at 85 % vVO2peak followed by 3-h monitored recovery. Venous blood samples were collected pre-, immediately post- and 3-h post-exercise, which were analyzed for interleukin-6, serum iron, ferritin and hepcidin.
Results
Interleukin-6 was elevated (p < 0.001) immediately post-exercise compared to baseline in both conditions, but was lower in HCHO (p = 0.015). Hepcidin levels were also lower at baseline (p = 0.049) in HCHO, and a large effect (d = 0.72) indicated a trend for lower levels at 3-h post-exercise compared to LCHO. Serum iron was increased post-exercise for both trials (p = 0.001), whereas serum ferritin remained unchanged.
Conclusions
Twenty-four hours of controlled low carbohydrate intake resulted in higher baseline hepcidin levels and post-exercise IL-6 responses than a high carbohydrate intake. Such hormone increases may be induced by gluconeogenic signaling of the liver, and may negatively impact an athlete’s iron metabolism.
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Abbreviations
- ANOVA:
-
Analysis of variance
- BLa:
-
Blood lactate
- cAMP:
-
Cyclic adenosine monophosphate
- CHO:
-
Carbohydrate
- CO2 :
-
Carbon dioxide
- CREBH:
-
cAMP response element-binding protein
- CV:
-
Coefficient of variation
- Fpn:
-
Ferroportin
- GXT:
-
Graded exercise test
- HAMP:
-
Hepcidin gene
- Hb:
-
Haemoglobin
- HCHO:
-
High carbohydrate trial
- Hct:
-
Haematocrit
- Hp:
-
Haptoglobin
- HR:
-
Heart rate
- IL-6:
-
Interleukin-6
- LCHO:
-
Low carbohydrate trial
- LSD:
-
Least significant difference
- O2 :
-
Oxygen
- PPARGG1A:
-
Peroxisome proliferator-activated receptor gamma coactivator 1 α
- RPE:
-
Rating of perceived exertion
- SD:
-
Standard deviation
- TLCH:
-
Train Low, Compete High
- VO2peak :
-
Peak oxygen uptake
- vVO2peak :
-
Velocity at peak oxygen uptake
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Acknowledgments
The authors wish to acknowledge the High Performance Sports Research Grant funding received from the Australian Sports Commission.
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Communicated by Fabio Fischetti.
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Badenhorst, C.E., Dawson, B., Cox, G.R. et al. Acute dietary carbohydrate manipulation and the subsequent inflammatory and hepcidin responses to exercise. Eur J Appl Physiol 115, 2521–2530 (2015). https://doi.org/10.1007/s00421-015-3252-3
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DOI: https://doi.org/10.1007/s00421-015-3252-3