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Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings

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Abstract

Plant cell walls undergo dynamic changes in response to different environmental stress conditions. In response to water deficit, two related proline-rich glycoproteins, called p33 and p36, accumulate in the soluble fraction of the cell walls in Phaseolus vulgaris (Covarrubias et al. in Plant Physiol 107:1119–1128, 1995). In this work, we show that p33 and p36 are able to form a 240 kDa oligomer, which is found in the cell wall soluble fraction. We present evidence indicating that the highest accumulation of these proteins in response to water deficit occurs in the growing regions of common bean seedlings, particularly in the phloem tissues. These proteins were detected in P. vulgaris cell suspension cultures, where the p33/p36 ratio was higher under hyperosmotic conditions than in bean seedlings subjected to the same treatment. The results support a role for these proteins during the plant cell response to changes in its water status, and suggest that cell wall modifications are induced in active growing cells of common bean in response to water limitation.

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Abbreviations

AP:

Alkaline phosphatase

dpa:

Days post-anthesis

PRP:

Proline-rich protein

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Acknowledgments

We thank J. Vázquez (Facultad de Química-UNAM) for allowing the use of the FPLC equipment in his laboratory and E. García-Ramírez (Facultad de Química-UNAM) for technical assistance in p33 and p36 protein purification. We are also grateful to J. L. Reyes and G. Cassab (Instituto de Biotecnologia-UNAM) for critical reading of the manuscript and stimulating discussions, to X. Alvarado and P. Rueda (Instituto de Biotecnologia-UNAM) for technical support in microscopy techniques and in the obtainment of the P. vulgaris cell-suspension culture, respectively, and to E. Mata (Instituto de Biotecnologia-UNAM) for animal care during the antibody production. S. Cuéllar and M. Battaglia were supported by a scholarship from DGEP-UNAM. This work was partially funded by grants from the Dirección General de Asuntos del Personal Académico (DGAPA-UNAM) IN204496 and CONACyT-México 40603-Q.

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  1. Magdalena Hernández

    Present address: Centro de Ciencias Genómicas, UNAM, Apdo. Postal 565-A, 62100, Cuernavaca, Morelos, Mexico

Authors and Affiliations

  1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250, Cuernavaca, Morelos, Mexico

    Marina Battaglia, Rosa M. Solórzano, Magdalena Hernández, Sonia Cuéllar-Ortiz, Blanca García-Gómez & Alejandra A. Covarrubias

  2. Laboratorio del Desarrollo en Plantas, Facultad de Ciencias, Ciudad Universitaria, Universidad Nacional Autónoma de México, 04510, Mexico D.F., Mexico

    Judith Márquez

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  1. Marina Battaglia
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Correspondence to Alejandra A. Covarrubias.

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Marina Battaglia and Rosa M. Solórzano contributed equally to this work.

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Battaglia, M., Solórzano, R.M., Hernández, M. et al. Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings. Planta 225, 1121–1133 (2007). https://doi.org/10.1007/s00425-006-0423-9

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